Journal ArticleDOI
Detection and analysis of mammary gland stem cells.
TLDR
Assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth are focused on.Abstract:
Emerging evidence from a variety of tissue types, including the mammary gland, suggests that normal stem and progenitor cells are the likely targets for malignant transformation, and that these transformed cells can function as cancer stem cells that drive tumour growth. In order to develop therapies that target these cancer stem cells, it is essential to determine the molecular mechanisms that regulate the growth and differentiation of these cells and their normal counterparts. To this end, a number of quantitative robust clonal assays have been developed that can detect the presence of human and mouse mammary stem and progenitor cells. These assays, when used in conjunction with cell-sorting strategies, have permitted the prospective isolation and characterization of a variety of cell types, including stem cells. Evidence to date indicates that these stem cells exhibit properties of basal mammary cells, possess extensive self-renewal properties, and are capable of generating a large number of phenotypically-distinct progenitor cells, many of which display characteristics of luminal cells. This review article will focus on the assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth.read more
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Journal ArticleDOI
Choosing the right cell line for breast cancer research
TL;DR: The issues surrounding the use of breast cancer cell lines as experimental models are discussed, in light of these revised clinical classifications, and suggestions for improving their use in translational breast cancer research are put forward.
Journal ArticleDOI
Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state
Christine L. Chaffer,Ines Brueckmann,Christina Scheel,Alicia J. Kaestli,Paul A. Wiggins,Leonardo O. Rodrigues,Mary W. Brooks,Ferenc Reinhardt,Ying Su,Kornelia Polyak,Lisa M. Arendt,Charlotte Kuperwasser,Brian Bierie,Robert A. Weinberg +13 more
TL;DR: It is demonstrated that normal and CSC-like cells can arise de novo from more differentiated cell types and that hierarchical models of mammary stem cell biology should encompass bidirectional interconversions between stem and nonstem compartments.
Journal ArticleDOI
Eyes wide open: a critical review of sphere-formation as an assay for stem cells.
TL;DR: A historical perspective of the evolution of the neurosphere assay is provided and limitations in the use of sphere-forming assays in the context of neurospheres are highlighted.
Journal ArticleDOI
Distinct stem cells contribute to mammary gland development and maintenance
Alexandra Van Keymeulen,Ana Rocha,Marielle Ousset,Benjamin Beck,Gaëlle Bouvencourt,Jason R. Rock,Neha Sharma,Sophie Dekoninck,Cédric Blanpain +8 more
TL;DR: In postnatal unperturbed mammary gland, both luminal and myoepithelial lineages contain long-lived unipotent stem cells that display extensive renewing capacities, as demonstrated by their ability to clonally expand during morphogenesis and adult life as well as undergo massive expansion during several cycles of pregnancy.
Journal ArticleDOI
Histological types of breast cancer: How special are they?
TL;DR: The associations between the molecular taxonomy of breast cancer and histological special types are reviewed, the possible origins of the heterogeneity of breast cancers are discussed, and an approach for the identification of novel therapeutic targets based on the study of histologicalspecial types of Breast cancer is proposed.
References
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Journal ArticleDOI
HER2 regulates the mammary stem/progenitor cell population driving tumorigenesis and invasion.
TL;DR: These studies provide support for the cancer stem cell hypothesis by suggesting that the effects of HER2 amplification on carcinogenesis, tumorigenesis and invasion may be due to its effects on normal and malignant mammary stem/progenitor cells.
Journal ArticleDOI
BRCA1 regulates human mammary stem/progenitor cell fate
Suling Liu,Christophe Ginestier,Emmanuelle Charafe-Jauffret,Hailey Foco,Celina G. Kleer,Sofia D. Merajver,Gabriela Dontu,Max S. Wicha +7 more
TL;DR: These studies demonstrate that BRCA1 plays a critical role in the differentiation of ER-negative stem/progenitor cells to ER-positive luminal cells and suggest that loss of BRC a1 may result in the accumulation of genetically unstable breast stem cells, providing prime targets for further carcinogenic events.
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Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
TL;DR: It is concluded that alpha 6 beta 4 in HFKs localizes in a new stable anchoring contact (SAC) that cooperates with alpha 3 beta 1- FAs to mediate adhesion to ECM, based on the following.
Journal ArticleDOI
An Atlas of Mouse Mammary Gland Development
TL;DR: A brief representation of the morphological changes that occur throughout the cycle of mouse mammary gland development is given so that developmental changes observed in mouse models of mammary development can be appreciated.
Journal ArticleDOI
Characterization of bipotent mammary epithelial progenitor cells in normal adult human breast tissue.
TL;DR: A hierarchical branching model of HBEC progenitor differentiation from a primitive uncommitted cell to luminal- and myoepithelial-restricted progenitors is supported.