Journal ArticleDOI
Detection and analysis of mammary gland stem cells.
TLDR
Assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth are focused on.Abstract:
Emerging evidence from a variety of tissue types, including the mammary gland, suggests that normal stem and progenitor cells are the likely targets for malignant transformation, and that these transformed cells can function as cancer stem cells that drive tumour growth. In order to develop therapies that target these cancer stem cells, it is essential to determine the molecular mechanisms that regulate the growth and differentiation of these cells and their normal counterparts. To this end, a number of quantitative robust clonal assays have been developed that can detect the presence of human and mouse mammary stem and progenitor cells. These assays, when used in conjunction with cell-sorting strategies, have permitted the prospective isolation and characterization of a variety of cell types, including stem cells. Evidence to date indicates that these stem cells exhibit properties of basal mammary cells, possess extensive self-renewal properties, and are capable of generating a large number of phenotypically-distinct progenitor cells, many of which display characteristics of luminal cells. This review article will focus on the assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth.read more
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Journal ArticleDOI
Choosing the right cell line for breast cancer research
TL;DR: The issues surrounding the use of breast cancer cell lines as experimental models are discussed, in light of these revised clinical classifications, and suggestions for improving their use in translational breast cancer research are put forward.
Journal ArticleDOI
Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state
Christine L. Chaffer,Ines Brueckmann,Christina Scheel,Alicia J. Kaestli,Paul A. Wiggins,Leonardo O. Rodrigues,Mary W. Brooks,Ferenc Reinhardt,Ying Su,Kornelia Polyak,Lisa M. Arendt,Charlotte Kuperwasser,Brian Bierie,Robert A. Weinberg +13 more
TL;DR: It is demonstrated that normal and CSC-like cells can arise de novo from more differentiated cell types and that hierarchical models of mammary stem cell biology should encompass bidirectional interconversions between stem and nonstem compartments.
Journal ArticleDOI
Eyes wide open: a critical review of sphere-formation as an assay for stem cells.
TL;DR: A historical perspective of the evolution of the neurosphere assay is provided and limitations in the use of sphere-forming assays in the context of neurospheres are highlighted.
Journal ArticleDOI
Distinct stem cells contribute to mammary gland development and maintenance
Alexandra Van Keymeulen,Ana Rocha,Marielle Ousset,Benjamin Beck,Gaëlle Bouvencourt,Jason R. Rock,Neha Sharma,Sophie Dekoninck,Cédric Blanpain +8 more
TL;DR: In postnatal unperturbed mammary gland, both luminal and myoepithelial lineages contain long-lived unipotent stem cells that display extensive renewing capacities, as demonstrated by their ability to clonally expand during morphogenesis and adult life as well as undergo massive expansion during several cycles of pregnancy.
Journal ArticleDOI
Histological types of breast cancer: How special are they?
TL;DR: The associations between the molecular taxonomy of breast cancer and histological special types are reviewed, the possible origins of the heterogeneity of breast cancers are discussed, and an approach for the identification of novel therapeutic targets based on the study of histologicalspecial types of Breast cancer is proposed.
References
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Journal ArticleDOI
Intrinsic Resistance of Tumorigenic Breast Cancer Cells to Chemotherapy
Xiaoxian Li,Michael T. Lewis,Jian Huang,Carolina Gutierrez,C. Kent Osborne,Meng Fen Wu,Susan G. Hilsenbeck,Anne Pavlick,Xiaomei Zhang,Gary C. Chamness,Helen Wong,Jeffrey M. Rosen,Jenny C. Chang +12 more
TL;DR: These studies provide clinical evidence for a subpopulation of chemotherapy-resistant breast cancer-initiating cells and, in combination with conventional therapy, specific pathway inhibitors may provide a therapeutic strategy for eliminating these cells to decrease recurrence and improve long-term survival.
Journal ArticleDOI
Purification and unique properties of mammary epithelial stem cells
John Stingl,Peter Eirew,Ian Ricketson,Mark Shackleton,François Vaillant,David H. Choi,Haiyan I. Li,Connie J. Eaves,Connie J. Eaves +8 more
TL;DR: The use of multi-parameter cell sorting and limiting dilution transplant analysis is reported to demonstrate the purification of a rare subset of adult mouse mammary cells that are able individually to regenerate an entire mammary gland within 6 weeks in vivo while simultaneously executing up to ten symmetrical self-renewal divisions.
Journal ArticleDOI
Granulocyte-Macrophage Progenitors as Candidate Leukemic Stem Cells in Blast-Crisis CML
Catriona Jamieson,Laurie Ailles,Scott J. Dylla,Manja Muijtjens,Carol D. Jones,James L. Zehnder,Jason Gotlib,Kevin Li,Markus G. Manz,Armand Keating,Charles L. Sawyers,Irving L. Weissman +11 more
TL;DR: Activation of beta-catenin in CML granulocyte-macrophage progenitors appears to enhance the self-renewal activity and leukemic potential of these cells.
Journal Article
Mutation selection and the natural history of cancer
TL;DR: The survival of the rapidly renewing tissues of long-lived animals like man requires that they be protected against the natural selection of fitter variant cells (that is, the spontaneous appearance of... as discussed by the authors.
Journal ArticleDOI
Transformation from committed progenitor to leukaemia stem cell initiated by MLL-AF9.
Andrei V. Krivtsov,David Twomey,David Twomey,Zhaohui Feng,Matthew C. Stubbs,Yingzi Wang,Joerg Faber,Jason E. Levine,Jing Wang,William C. Hahn,William C. Hahn,D. Gary Gilliland,D. Gary Gilliland,Todd R. Golub,Todd R. Golub,Scott A. Armstrong +15 more
TL;DR: It is shown that leukaemia stem cells (LSC) can maintain the global identity of the progenitor from which they arose while activating a limited stem-cell- or self-renewal-associated programme.