Journal ArticleDOI
Detection and analysis of mammary gland stem cells.
TLDR
Assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth are focused on.Abstract:
Emerging evidence from a variety of tissue types, including the mammary gland, suggests that normal stem and progenitor cells are the likely targets for malignant transformation, and that these transformed cells can function as cancer stem cells that drive tumour growth. In order to develop therapies that target these cancer stem cells, it is essential to determine the molecular mechanisms that regulate the growth and differentiation of these cells and their normal counterparts. To this end, a number of quantitative robust clonal assays have been developed that can detect the presence of human and mouse mammary stem and progenitor cells. These assays, when used in conjunction with cell-sorting strategies, have permitted the prospective isolation and characterization of a variety of cell types, including stem cells. Evidence to date indicates that these stem cells exhibit properties of basal mammary cells, possess extensive self-renewal properties, and are capable of generating a large number of phenotypically-distinct progenitor cells, many of which display characteristics of luminal cells. This review article will focus on the assays used to detect mammary stem and progenitor cells, some of the properties of these cells and their progeny and how they relate to the cancer stem cells that drive breast tumour growth.read more
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Journal ArticleDOI
Choosing the right cell line for breast cancer research
TL;DR: The issues surrounding the use of breast cancer cell lines as experimental models are discussed, in light of these revised clinical classifications, and suggestions for improving their use in translational breast cancer research are put forward.
Journal ArticleDOI
Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state
Christine L. Chaffer,Ines Brueckmann,Christina Scheel,Alicia J. Kaestli,Paul A. Wiggins,Leonardo O. Rodrigues,Mary W. Brooks,Ferenc Reinhardt,Ying Su,Kornelia Polyak,Lisa M. Arendt,Charlotte Kuperwasser,Brian Bierie,Robert A. Weinberg +13 more
TL;DR: It is demonstrated that normal and CSC-like cells can arise de novo from more differentiated cell types and that hierarchical models of mammary stem cell biology should encompass bidirectional interconversions between stem and nonstem compartments.
Journal ArticleDOI
Eyes wide open: a critical review of sphere-formation as an assay for stem cells.
TL;DR: A historical perspective of the evolution of the neurosphere assay is provided and limitations in the use of sphere-forming assays in the context of neurospheres are highlighted.
Journal ArticleDOI
Distinct stem cells contribute to mammary gland development and maintenance
Alexandra Van Keymeulen,Ana Rocha,Marielle Ousset,Benjamin Beck,Gaëlle Bouvencourt,Jason R. Rock,Neha Sharma,Sophie Dekoninck,Cédric Blanpain +8 more
TL;DR: In postnatal unperturbed mammary gland, both luminal and myoepithelial lineages contain long-lived unipotent stem cells that display extensive renewing capacities, as demonstrated by their ability to clonally expand during morphogenesis and adult life as well as undergo massive expansion during several cycles of pregnancy.
Journal ArticleDOI
Histological types of breast cancer: How special are they?
TL;DR: The associations between the molecular taxonomy of breast cancer and histological special types are reviewed, the possible origins of the heterogeneity of breast cancers are discussed, and an approach for the identification of novel therapeutic targets based on the study of histologicalspecial types of Breast cancer is proposed.
References
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Journal ArticleDOI
Human breast cancer stem cell markers CD44 and CD24: enriching for cells with functional properties in mice or in man?
TL;DR: Understanding whether CD44+/CD24- breast cancer cells are merely more successful in overcoming an engraftment incompatibility that exists when injecting human cells into the mouse adipose tissue or are indeed bona fide cancer stem cells is of great importance.
Journal ArticleDOI
Re-evaluation of mammary stem cell biology based on in vivo transplantation.
Gilbert H. Smith,Daniel Medina +1 more
TL;DR: Re-examination of the literature on the transplantation biology of mammary gland regeneration reveals that a complex, combinatorial interaction between variously differentiated mammary epithelial cells and the mammary fat pad stroma is indispensable to this process.
Journal ArticleDOI
“Destemming” Cancer Stem Cells
Richard P. Hill,Roberto Perris +1 more
TL;DR: Interest in cancer stem cells is currently high, arising from recent reports identifying cell surface markers that can be used to sort such cells from primary human tumors, but use of the term cancer stem cell may be misleading.
Journal ArticleDOI
Highly purified primitive hematopoietic stem cells are PML-RARA negative and generate nonclonal progenitors in acute promyelocytic leukemia
Ali G. Turhan,François M. Lemoine,C Debert,M L Bonnet,Claude Baillou,F Picard,Elisabeth Macintyre,Bruno Varet +7 more
TL;DR: It is suggested that the primitive hematopoietic stem cells as defined by the CD34+/CD38- antigens are not involved by the neoplastic process in APL, and may have important implications for autografting strategies of retinoic acid/chemotherapy-resistant or relapsed patients.
Journal ArticleDOI
Reconstruction of human mammary tissues in a mouse model
TL;DR: The human-in-mouse model has the potential to improve the understanding of crosstalk between tissue stroma and the epithelium as well as factors involved in breast stem cell biology tumor initiation and progression.