Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome
TLDR
All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromaatin and with the isoform.Abstract:
The pattern of histone H4 acetylation in different genomic regions has been investigated by immunoprecipitating oligonucleosomes from a human lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8, 12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot blotting. Pol I and pol II transcribed genes located in euchromatin were shown to have levels of H4 acetylation at lysines 5, 8 and 12 equivalent to those in input chromatin, but to be slightly enriched in H4 acetylated at lysine 16. In no case did the acetylation level correlate with actual or potential transcriptional activity. All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromatin and with the isoform. Two single copy genes that map within or adjacent to blocks of paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8 and 12, but not 16. Consensus sequences of repetitive elements of the Alu family (SINES, enriched in R bands) were associated with H4 that was more highly acetylated at all four lysines than input chromatin, while H4 associated with Kpn I elements (LINES, enriched in G bands) was significantly underacetylated.read more
Citations
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Journal ArticleDOI
Cell-line specific chromatin acetylation at the Sox10-Pax3 enhancer site modulates the RET proto-oncogene expression.
Francesca Puppo,Paola Griseri,Mirco Fanelli,Francesca Schena,Giovanni Romeo,Pier Giuseppe Pelicci,Isabella Ceccherini,Roberto Ravazzolo,Giovanna Patrone +8 more
TL;DR: It is shown that the histone deacetylase inhibitor sodium butyrate increases RET transcription in cells displaying low levels of its mRNA, while it has no effect in cells expressing at high levels.
Journal ArticleDOI
Active transgenes in zebrafish are enriched in acetylated histone H4 and dynamically associate with RNA Pol II and splicing complexes
TL;DR: A correlation between H4 acetylation and transgene activity is demonstrated, and it is argued that active transgenes dynamically recruit splicing factors and Pol II and the dynamic association of the transgene with splicing factor SC35 and RNA Pol II is demonstrated.
Journal ArticleDOI
Immunological analysis of chromatin: FIS and CHIPS
Journal ArticleDOI
Condensation patterns of prophase/prometaphase chromosome are correlated with H4K5 histone acetylation and genomic DNA contents in plants
TL;DR: The data indicate that theCP of a plant chromosome complement is influenced but not exclusively determined by nuclear and chromosomal DNA contents, whereas the CP of individual chromosomes is clearly correlated with H4K5ac distribution.
Journal ArticleDOI
A human genomic library enriched in transcriptionally active sequences (aDNA library).
TL;DR: The aDNA library is very gene rich and not skewed toward the most highly expressed sequences, as in mRNA libraries, and could be a valuable source of such sequences, particularly those that lack CpG islands or other features that allow their specific selection.
References
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