Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome
TLDR
All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromaatin and with the isoform.Abstract:
The pattern of histone H4 acetylation in different genomic regions has been investigated by immunoprecipitating oligonucleosomes from a human lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8, 12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot blotting. Pol I and pol II transcribed genes located in euchromatin were shown to have levels of H4 acetylation at lysines 5, 8 and 12 equivalent to those in input chromatin, but to be slightly enriched in H4 acetylated at lysine 16. In no case did the acetylation level correlate with actual or potential transcriptional activity. All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromatin and with the isoform. Two single copy genes that map within or adjacent to blocks of paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8 and 12, but not 16. Consensus sequences of repetitive elements of the Alu family (SINES, enriched in R bands) were associated with H4 that was more highly acetylated at all four lysines than input chromatin, while H4 associated with Kpn I elements (LINES, enriched in G bands) was significantly underacetylated.read more
Citations
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Journal ArticleDOI
Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter
TL;DR: It is found that the 1α,25(OH)2D3 inducibility of cyclin C mRNA expression, in relationship with the 24-hydroxylase (CYP24) gene, was best in MCF-7 human breast cancer cells.
Journal ArticleDOI
Genomic Sequence and Transcriptional Profile of the Boundary Between Pericentromeric Satellites and Genes on Human Chromosome Arm 10p
J. Guy,Tom Hearn,Moira Crosier,Jonathan M. Mudge,Luigi Viggiano,Dirk Koczan,Hans Jürgen Thiesen,Jeffrey A. Bailey,Julie E. Horvath,Evan E. Eichler,Mark E. Earthrowl,Panos Deloukas,Lisa French,Jane Rogers,David Bentley,Michael S. Jackson +15 more
TL;DR: It is indicated that recent interchromosomal duplications at this centromere have involved transcriptionally inert, satellite rich DNA, which is likely to be heterochromatic, which suggests that any novel gene structures formed by these inter chromosomal events would require relocation to a more open chromatin environment to be expressed.
Journal ArticleDOI
Genomic sequence and transcriptional profile of the boundary between pericentromeric satellites and genes on human chromosome arm 10q.
J. Guy,Cosma Spalluto,A. McMurray,Tom Hearn,Moira Crosier,Luigi Viggiano,Valeria Miolla,Nicoletta Archidiacono,Mariano Rocchi,C. Scott,P.A. Lee,J. Sulston,Jeffrey Rogers,David Bentley,Michael S. Jackson +14 more
TL;DR: 1025 kb of contiguous human genomic sequence is presented which links pericentromeric satellites to the RET proto-oncogene in 10q11.2 and is presumed to span the transition from centric heterochromatin to euchromatin on this chromosome arm.
Book ChapterDOI
Regulating Chromatin by Histone Acetylation
TL;DR: The functions of protein complexes involved in the addition and the removal of this mark on chromatin and how it is recognized as a signal are covered and how acetylation is functionally linked to other histone modifications are presented.
Journal ArticleDOI
An integrated biological approach to nuclear receptor signaling in physiological control and disease.
TL;DR: The fine regulation of this NR network is specific to each human individual and depends, in part, on the constellation of regulatory small nucleotide polymorphisms (SNPs) in his or her genome.
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