Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome
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TLDR
All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromaatin and with the isoform.Abstract:
The pattern of histone H4 acetylation in different genomic regions has been investigated by immunoprecipitating oligonucleosomes from a human lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8, 12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot blotting. Pol I and pol II transcribed genes located in euchromatin were shown to have levels of H4 acetylation at lysines 5, 8 and 12 equivalent to those in input chromatin, but to be slightly enriched in H4 acetylated at lysine 16. In no case did the acetylation level correlate with actual or potential transcriptional activity. All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromatin and with the isoform. Two single copy genes that map within or adjacent to blocks of paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8 and 12, but not 16. Consensus sequences of repetitive elements of the Alu family (SINES, enriched in R bands) were associated with H4 that was more highly acetylated at all four lysines than input chromatin, while H4 associated with Kpn I elements (LINES, enriched in G bands) was significantly underacetylated.read more
Citations
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Testing the position-effect variegation hypothesis for facioscapulohumeral muscular dystrophy by analysis of histone modification and gene expression in subtelomeric 4q
Guanchao Jiang,Fan Yang,Petra G.M. van Overveld,Vettaikorumakankav Vedanarayanan,Silvère M. van der Maarel,Melanie Ehrlich +5 more
TL;DR: The results favor a new model for the molecular genetic etiology of FSHD, such as, differential long-distance cis looping that depends upon the presence of a 4q35 D4Z4 array with less than a threshold number of copies of the 3.3 kb repeat.
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DNA methylation is linked to deacetylation of histone H3, but not H4, on the imprinted genes Snrpn and U2af1-rs1.
Richard I. Gregory,Tamzin E. Randall,Colin A. Johnson,Sanjeev Khosla,Izuho Hatada,Laura P. O'Neill,Bryan M. Turner,Robert Feil,Robert Feil +8 more
TL;DR: Data from ChIP and resolution of parental alleles using single-strand conformational polymorphisms are consistent with the hypothesis that CpG methylation leads to deacetylation of histone H3, but not H4, through a process that involves selective binding of MBD proteins.
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Spatio-temporal activation of chromatin on the human CYP24 gene promoter in the presence of 1alpha,25-Dihydroxyvitamin D3.
TL;DR: New information is revealed concerning the regulation of the CYP24 gene by 1alpha,25OH2D3, and is a demonstration of the simultaneous participation of multiple, structurally diverse response elements in promoter activation in a living cell.
Journal ArticleDOI
A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere
Anthony W.I. Lo,Jeffrey M. Craig,Richard Saffery,Paul Kalitsis,Danielle V. Irvine,Elizabeth D. Earle,Dianna J. Magliano,K. H. Andy Choo +7 more
TL;DR: A 330 kb CENP‐A binding domain is defined of a 10q25.3 neocentromere found on the human marker chromosome mardel(10), providing evidence for the replacement of histone H3 by CENp‐A within centromere‐specific nucleosomes.
Journal ArticleDOI
Histone H4 lysine 20 monomethylation is increased in promoter and coding regions of active genes and correlates with hyperacetylation
TL;DR: The chromatin immunopre-cipitation results demonstrated that in contrast to trimethylated H4-Lys20, which was found to inversely correlate with H4 hyper-acetylation, H 4-LYS20 monomethylation is compatible with histone H4Hyperacetylated and correlates with the transcriptionally active or competent chromatin state.
References
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