Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome
TLDR
All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromaatin and with the isoform.Abstract:
The pattern of histone H4 acetylation in different genomic regions has been investigated by immunoprecipitating oligonucleosomes from a human lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8, 12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot blotting. Pol I and pol II transcribed genes located in euchromatin were shown to have levels of H4 acetylation at lysines 5, 8 and 12 equivalent to those in input chromatin, but to be slightly enriched in H4 acetylated at lysine 16. In no case did the acetylation level correlate with actual or potential transcriptional activity. All acetylated histone H4 isoforms were depleted in non-coding, simple repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromatin and with the isoform. Two single copy genes that map within or adjacent to blocks of paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8 and 12, but not 16. Consensus sequences of repetitive elements of the Alu family (SINES, enriched in R bands) were associated with H4 that was more highly acetylated at all four lysines than input chromatin, while H4 associated with Kpn I elements (LINES, enriched in G bands) was significantly underacetylated.read more
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Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies
Yoko Hayashi-Takanaka,Yoko Hayashi-Takanaka,Kazumitsu Maehara,Akihito Harada,Takashi Umehara,Shigeyuki Yokoyama,Chikashi Obuse,Yasuyuki Ohkawa,Naohito Nozaki,Hiroshi Kimura,Hiroshi Kimura +10 more
TL;DR: These extensively characterized and highly specific antibodies will be useful for future epigenetics and epigenome studies and Chromatin immunoprecipiation combined with deep sequencing revealed that acetylation of both H4K8 and H 4K16 were enriched around transcription start sites.
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Characterization of neurohistone variants and post-translational modifications by electron capture dissociation mass spectrometry
TL;DR: The first mass spectrometric look at histone variant expression and post-translational modifications from histones isolated from rat brain tissue is taken, in honor of Professor Donald F. Hunt.
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Frequent aberrant methylation of the imprinted IGF2/H19 locus and LINE1 hypomethylation in ovarian carcinoma
Reinhard Dammann,Sebastian Kirsch,Undraga Schagdarsurengin,Temuujin Dansranjavin,Elise Gradhand,Wolfgang D. Schmitt,Steffen Hauptmann +6 more
TL;DR: Frequent combined aberrant methylation of the IGF2-H19 locus and LINE1 is found in the vast majority of OC, suggesting that these changes are important events in tumorigenesis.
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Epigenetik – ein Epizentrum der Genregulation: Histone und histonmodifizierende Enzyme
Journal ArticleDOI
Euchromatic domains in plant chromosomes as revealed by H4 histone acetylation and early DNA replication.
TL;DR: There are no detectable differences in intensity and distribution of H4 acetylation between these two X chromosomes in Silene latifolia, and the presented data indicate that Silene species possess the specific distal-subtelomeric location of euchromatin, gene-rich regions on chromosomes.
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