Journal ArticleDOI
Domain shapes, coarsening, and random patterns in ternary membranes.
Reads0
Chats0
TLDR
Analysis of the spatial domain pattern in terms of Voronoi polygons demonstrates a close similarity to equilibrated cellular structures with a maximized configurational entropy.Abstract:
A number of morphological and statistical aspects of domain formation in singly and doubly supported ternary membranes have been investigated. Such ternary membranes produce macroscopic phase separation in two fluid phases and are widely used as raft models. We find that membrane interactions with the support surface can have a critical influence on the domain shapes if measures are not taken to screen these interactions. Combined AFM and fluorescence microscopy demonstrate small (500 nm) irregular domains and incomplete formation of much larger (5 μm) round domains. These kinetically trapped structures are the result of interactions between the membrane and the support surface, and they can be effectively removed by employing doubly supported membranes under physiological salt concentrations. These decoupled supported membranes display macroscopic round domains that are easily perturbed by fluid shear flow. The system allows a quantitative characterization of domain coarsening upon being cooled into the ...read more
Citations
More filters
Journal ArticleDOI
Shiga toxin induces tubular membrane invaginations for its uptake into cells
Winfried Römer,Winfried Römer,Ludwig Berland,Valérie Chambon,Valérie Chambon,Katharina Gaus,Barbara Windschiegl,Danièle Tenza,Mohamed R. E. Aly,Vincent Fraisier,Jean-Claude Florent,David Perrais,Christophe Lamaze,Christophe Lamaze,Graça Raposo,Claudia Steinem,Pierre Sens,Patricia Bassereau,Ludger Johannes,Ludger Johannes +19 more
TL;DR: It is shown that the Gb3 (glycolipid)-binding B-subunit of bacterial Shiga toxin induces narrow tubular membrane invaginations in human and mouse cells and model membranes, and it is concluded that the B- subunit induces lipid reorganization that favours negative membrane curvature, which drives the formation of inward membrane tubules.
Journal ArticleDOI
Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment
Alf Honigmann,Geert van den Bogaart,Geert van den Bogaart,Emilio Iraheta,H. Jelger Risselada,Dragomir Milovanovic,Veronika Mueller,Stefan Müllar,Ulf Diederichsen,Dirk Fasshauer,Helmut Grubmüller,Stefan W. Hell,Christian Eggeling,Christian Eggeling,Karin Kühnel,Reinhard Jahn +15 more
TL;DR: The results suggest that PIP2 clusters organized by syntaxin-1 act as molecular beacons for vesicle docking, with the subsequent Ca2+ influx bringing the vesicles membrane close enough for membrane fusion.
Journal ArticleDOI
An outlook on organization of lipids in membranes: searching for a realistic connection with the organization of biological membranes.
TL;DR: Results from a number of experimental and theoretical studies of model lipid bilayers are reviewed, emphasizing the significance of these fundamental physical properties for the structure and dynamics of biological membranes.
Journal ArticleDOI
Phase diagrams of lipid mixtures relevant to the study of membrane rafts
Félix M. Goñi,Alicia Alonso,Luis A. Bagatolli,Rhoderick E. Brown,Derek Marsh,Manuel Prieto,Jenifer Thewalt +6 more
TL;DR: The present paper reviews the phase properties of phosphatidylcholine-sphingomyelin-cholesterol mixtures, that are often used as models for membrane "raft" microdomains, and critically evaluated and rationalized in terms of triangular phase diagrams.
Journal ArticleDOI
A lipid bound actin meshwork organizes liquid phase separation in model membranes.
TL;DR: In this article, the authors used computer simulations and two advanced techniques (super-resolution optical STED microscopy and fluorescence correlation spectroscopy) to explore the properties of a model membrane in the presence of a dense network of actin fibers.
References
More filters
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
The kinetics of precipitation from supersaturated solid solutions
I.M. Lifshitz,V.V. Slyozov +1 more
TL;DR: In this paper, an analysis is made of the process whereby diffusion effects can cause the precipitation of grains of a second phase in a supersaturated solid solution, and the kinetics of this type of grain growth are examined in detail.
Journal ArticleDOI
Films Built by Depositing Successive Monomolecular Layers on a Solid Surface
TL;DR: Arbor as mentioned in this paper showed that xanthone and lluorenone possess new bands in sulfuric acid sohtions which are associated with the stabilization of the quinonoid structure for these compounds.
Journal ArticleDOI
Lipid Rafts: Elusive or Illusive?
TL;DR: There has been considerable recent interest in the possibility that the plasma membrane contains lipid "rafts," microdomains enriched in cholesterol and sphingolipids, and it seems that a definitive proof of raft existence has yet to be obtained.
Journal ArticleDOI
Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.
Sarah L. Veatch,Sarah L. Keller +1 more
TL;DR: In this article, the authors use fluorescence microscopy to directly observe liquid phases in giant unilamellar vesicles and find a simple relationship between chain melting temperature and miscibility transition temperature that holds for both phosphatidylcholine and sphingomyelin lipids.
Related Papers (5)
Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.
Sarah L. Veatch,Sarah L. Keller +1 more