Journal ArticleDOI
Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides.
Kenneth C. Burtis,Bruce S. Baker +1 more
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TLDR
Genetic and molecular data suggest that sequences including and adjacent to the female-specific splice acceptor site play an important role in the regulation of dsx expression by the transformer and transformer-2 loci.About:
This article is published in Cell.The article was published on 1989-03-24. It has received 642 citations till now. The article focuses on the topics: DM domain & Doublesex.read more
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Sorting out the complexity of SR protein functions.
TL;DR: This review discusses recent advances in understanding the diverse functions of SR proteins in metazoan pre-mRNA splicing and presents a model that takes these new findings into account.
Journal ArticleDOI
A new protease required for cell-cycle progression in yeast
Shyr-Jiann Li,Mark Hochstrasser +1 more
TL;DR: A yeast Ubl-specific protease, Ulp1, which cleaves proteins from Smt3 and SUMO-1 but not from ubiquitin is described, indicating the existence of a widely conserved protease fold.
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Evidence for evolutionary conservation of sex-determining genes
Christopher S. Raymond,Caroline E. Shamu,Caroline E. Shamu,Michael M. Shen,Michael M. Shen,Kelly J. Seifert,Betsy A. Hirsch,Jonathan Hodgkin,David Zarkower +8 more
TL;DR: The male sexual regulatory gene mab-3 from the nematode Caenorhabditis elegans is isolated and found that it is related to the Drosophila melanogasterSexual regulatory gene doublesex (dsx), which encodes proteins with a DNA-binding motif that is named the ‘DM domain’.
Journal ArticleDOI
A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes
Kyros Kyrou,Andrew Hammond,Roberto Galizi,Nace Kranjc,Austin Burt,Andrea Beaghton,Tony Nolan,Andrea Crisanti +7 more
TL;DR: It is found that CRISPR–Cas9-targeted disruption of the intron 4–exon 5 boundary aimed at blocking the formation of functional AgdsxF did not affect male development or fertility, whereas females homozygous for the disrupted allele showed an intersex phenotype and complete sterility.
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VIVE LA DIFFÉRENCE:Males vs Females in Flies vs Worms
Thomas W. Cline,Barbara J Meyer +1 more
TL;DR: This review compares and contrast the strategies used for sex determination between "the fly" and "the worm" and the way this understanding has come about and striking similarities have been found in the genetic strategies used by these two species to differentiate their sexes.
References
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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Improved tools for biological sequence comparison.
TL;DR: Three computer programs for comparisons of protein and DNA sequences can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.
Journal ArticleDOI
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
Journal ArticleDOI
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
TL;DR: A method is described for the rapid generation and cloning of deletion derivatives well-suited for the sequencing of long stretches of DNA based on two useful features of exonuclease III: processive digestion at a very uniform rate and failure to initiate digestion at DNA ends with four-base 3'-protrusions.
Related Papers (5)
Sex and the single cell. i. on the action of major loci affecting sex determination in drosophila melanogaster
Bruce S. Baker,Kimberly A. Ridge +1 more