Dynein motion switches from diffusive to directed upon cortical anchoring.
Vaishnavi Ananthanarayanan,Martin H. Schattat,Sven Vogel,Alexander Krull,Nenad Pavin,Nenad Pavin,Iva Marija Tolić-Norrelykke +6 more
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TLDR
It is observed that dyneins on the microtubule move either in a diffusive or directed manner, with the switch from diffusion to directed movement occurring upon binding of dynein to cortical anchors.About:
This article is published in Cell.The article was published on 2013-06-20 and is currently open access. It has received 89 citations till now. The article focuses on the topics: Cytoplasmic Dyneins & Dynactin.read more
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Functions and mechanics of dynein motor proteins
TL;DR: A model for the mechanochemical cycle of dynein is emerging, in which nucleotide-driven flexing motions within the AAA+ ring of Dynein alter the affinity of its microtubule-binding stalk and reshape its mechanical element to generate movement.
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Probing the Stochastic, Motor-Driven Properties of the Cytoplasm Using Force Spectrum Microscopy
Ming Guo,Allen J. Ehrlicher,Allen J. Ehrlicher,Mikkel H. Jensen,Mikkel H. Jensen,Malte Renz,Jeffrey R. Moore,Robert D. Goldman,Jennifer Lippincott-Schwartz,Fred C. MacKintosh,David A. Weitz +10 more
TL;DR: This work introduces force-spectrum-microscopy (FSM) to directly quantify random forces within the cytoplasm of cells and thereby probe stochastic motor activity, and shows that force fluctuations substantially enhance intracellular movement of small and large components.
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Mechanism and regulation of cytoplasmic dynein.
TL;DR: This work provides a synthesis of recent data to describe the current model of dynein's mechanochemical cycle and discusses regulators of Dynein, with particular focus on those that directly interact with the motor to modulate its recruitment to microtubules, initiate cargo transport, or activate minus-end-directed motility.
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Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein
Takayuki Torisawa,Muneyoshi Ichikawa,Akane Furuta,Kei Saito,Kazuhiro Oiwa,Hiroaki Kojima,Yoko Y. Toyoshima,Ken'ya Furuta +7 more
TL;DR: A mechanism of autonomous on–off switching of cargo transport is proposed, in which single dynein molecules in the cell are autoinhibited through intramolecular head–head stacking and become active when they assemble as a team on a cargo.
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Local Cytoskeletal and Organelle Interactions Impact Molecular-Motor-Driven Early Endosomal Trafficking
TL;DR: The results indicate that the crowded cellular environment significantly impacts the motor-driven motility of organelles and suggest that endosomal membrane tension is high during directed run termination.
References
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Jürg Bähler,Jian-Qiu Wu,Mark S. Longtine,Nirav Shah,Amos Mckenzie,Alexander B. Steever,Achim Wach,Peter Philippsen,John R. Pringle +8 more
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Single-Molecule Analysis of Dynein Processivity and Stepping Behavior
Samara L. Reck-Peterson,Ahmet Yildiz,Andrew P. Carter,Arne Gennerich,Nan Zhang,Ronald D. Vale +5 more
TL;DR: The results suggest that cytoplasmic dynein moves processively through the coordination of its two motor domains, but its variable step size and direction suggest a considerable diffusional component to its step, which differs from Kinesin-1 and is more akin to myosin VI.