Easy Fabrication of Thin Membranes with Through Holes. Application to Protein Patterning

TLDR: This paper presents a simple, rapid and reliable technique to fabricate thin layers of UV curable polymer with through holes that allows highly reproducible and highly homogeneous protein patterning with micron sized resolution on surfaces as large as 10 cm2.

Abstract: Since protein patterning on 2D surfaces has emerged as an important tool in cell biology, the development of easy patterning methods has gained importance in biology labs In this paper we present a simple, rapid and reliable technique to fabricate thin layers of UV curable polymer with through holes These membranes are as easy to fabricate as microcontact printing stamps and can be readily used for stencil patterning We show how this microfabrication scheme allows highly reproducible and highly homogeneous protein patterning with micron sized resolution on surfaces as large as 10 cm2 Using these stencils, fragile proteins were patterned without loss of function in a fully hydrated state We further demonstrate how intricate patterns of multiple proteins can be achieved by stacking the stencil membranes We termed this approach microserigraphy