Exported Proteins Required for Virulence and Rigidity of Plasmodium falciparum-Infected Human Erythrocytes
Alexander G. Maier,Melanie Rug,Matthew T. O'Neill,Monica Brown,Srabasti J. Chakravorty,Tadge Szestak,Joanne M. Chesson,Yang Wu,Katie R. Hughes,Ross L. Coppel,Chris I. Newbold,James G. Beeson,Alister Craig,Brendan S. Crabb,Alan F. Cowman +14 more
TLDR
It is shown that multiple proteins play a role in generating increased rigidity of infected erythrocytes and may provide targets for antivirulence based therapies to a disease responsible for millions of deaths annually.About:
This article is published in Cell.The article was published on 2008-07-11 and is currently open access. It has received 483 citations till now. The article focuses on the topics: KAHRP & Plasmodium falciparum.read more
Citations
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A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Saima M. Sidik,Diego Huet,Suresh M. Ganesan,My Hang Huynh,Timothy C. Wang,Timothy C. Wang,Armiyaw S. Nasamu,Prathapan Thiru,Jeroen P. J. Saeij,Vern B. Carruthers,Jacquin C. Niles,Sebastian Lourido +11 more
TL;DR: In this article, the authors adapted CRISPR/Cas9 to assess the contribution of each gene from the parasite Toxoplasma gondii during infection of human fibroblasts.
Journal ArticleDOI
Cell-Cell Communication between Malaria-Infected Red Blood Cells via Exosome-like Vesicles
Neta Regev-Rudzki,Danny W. Wilson,Danny W. Wilson,Teresa Carvalho,Teresa Carvalho,Xavier Sisquella,Xavier Sisquella,Bradley M. Coleman,Melanie Rug,Melanie Rug,Dejan Bursac,Dejan Bursac,Fiona Angrisano,Fiona Angrisano,Michelle L. Gee,Andrew F. Hill,Jake Baum,Jake Baum,Alan F. Cowman,Alan F. Cowman +19 more
TL;DR: This study reveals a previously unidentified pathway of P. falciparum biology critical for survival in the host and transmission to mosquitoes and identifies a pathway for the development of agents to block parasite transmission from the human host to the mosquito.
A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes
Saima M. Sidik,Diego Huet,My Hang Huynh,Prathapan Thiru,Jeroen P. J. Saeij,Vern B. Carruthers,Sebastian Lourido,Suresh M. Ganesan,Armiyaw S. Nasamu,Jacquin C. Niles,Timothy C. Wang +10 more
TL;DR: The first genome-wide genetic screen of an apicomplexan parasite is presented, revealing essential functions during infection of human cells and providing broad-based functional information on T. gondii genes that will facilitate future approaches to expand the horizon of antiparasitic interventions.
Journal ArticleDOI
Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes
Ellen Bushell,Ana Rita Gomes,Theo Sanderson,Burcu Anar,Gareth Girling,Colin Herd,Tom Metcalf,Katarzyna Modrzynska,Frank Schwach,Rowena E. Martin,Michael W. Mather,Geoffrey I. McFadden,Leopold Parts,Gavin G. Rutledge,Akhil B. Vaidya,Kai Wengelnik,Julian C. Rayner,Oliver Billker +17 more
TL;DR: The genomes of malaria parasites contain many genes of unknown function and the level of genetic redundancy in a single-celled organism may reflect the degree of environmental variation it experiences, which helps rationalize both the relative successes of drugs and the greater difficulty of making an effective vaccine.
Journal ArticleDOI
A newly discovered protein export machine in malaria parasites
Tania F. de Koning-Ward,Tania F. de Koning-Ward,Paul R. Gilson,Paul R. Gilson,Justin A Boddey,Melanie Rug,Brian J. Smith,Anthony T. Papenfuss,Paul R. Sanders,Rachel J. Lundie,Alexander G. Maier,Alan F. Cowman,Brendan S. Crabb,Brendan S. Crabb +13 more
TL;DR: This work has identified in Plasmodium falciparum a translocon of exported proteins (PTEX), which is located in the vacuole membrane and offers a new avenue for therapeutic intervention.
References
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The Transcriptome of the Intraerythrocytic Developmental Cycle of Plasmodium falciparum
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The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of plasmodium falciparum-infected erythrocytes
Xin-zhuan Su,Virginia M. Heatwole,Samuel P. Wertheimer,Frangoise Guinet,Jacqueline A. Herrfeldt,David S. Peterson,Jeffrey A. Ravetch,Thomas E. Wellems +7 more
TL;DR: A large and extremely diverse family of P. falciparum genes (var) that encode 200-350 kDa proteins having the expected properties of antigenically variant adhesion molecules are described.
Journal ArticleDOI
Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes
Dror I. Baruch,Britten L. Pasloske,Hardeep B. Singh,Xiahui Bi,Xin C. Ma,Michael Feldman,Theodore F. Taraschi,Russell J. Howard +7 more
TL;DR: The cloning of two related PfEMP1 genes from the Malayan Camp parasite strain are described and the molecular basis for antigenic variation in malaria and adherence of infected erythrocytes to host cells can now be pursued.
Journal ArticleDOI
Switches in expression of Plasmodium falciparum var genes correlate with changes in antigenic and cytoadherent phenotypes of infected erythrocytes.
Joseph D. Smith,Chetan E. Chitnis,Alistar G. Craig,David J. Roberts,Diana E. Hudson-Taylor,David S. Peterson,Robert Pinches,Chris I. Newbold,Louis H. Miller +8 more
TL;DR: It is shown that expression of variant antigenic determinants is correlated with expression of individual members of a large, multigene family named var, which is consistent with the involvement of var genes in antigenic variation and binding to endothelium.