Journal ArticleDOI
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
Clifford P. Brangwynne,Christian R. Eckmann,David S. Courson,Agata Rybarska,Carsten Hoege,Jöbin Gharakhani,Jöbin Gharakhani,Frank Jülicher,Frank Jülicher,Anthony A. Hyman,Anthony A. Hyman +10 more
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TLDR
It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell.Abstract:
In sexually reproducing organisms, embryos specify germ cells, which ultimately generate sperm and eggs In Caenorhabditis elegans, the first germ cell is established when RNA and protein-rich P granules localize to the posterior of the one-cell embryo Localization of P granules and their physical nature remain poorly understood Here we show that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which we used to estimate their viscosity and surface tension As with other liquids, P granules rapidly dissolved and condensed Localization occurred by a biased increase in P granule condensation at the posterior This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasmread more
Citations
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Theory of droplet ripening in stiffness gradients
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Higher-order assemblies in immune signaling: supramolecular complexes and phase separation.
TL;DR: A review of higher-order assemblies in major immune pathways can be found in this article, where the authors discuss how their structural organization enables elegant mechanisms of signaling regulation and discuss how these structures enable elegant mechanisms for immune regulation.
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Structural Insight into IAPP-Derived Amyloid Inhibitors and Their Mechanism of Action
Zheng Niu,Elke Prade,Eleni Malideli,Kathleen Hille,Alexander Jussupow,Alexander Jussupow,Yonatan G. Mideksa,Yonatan G. Mideksa,Li-Mei Yan,Chen Qian,Markus Fleisch,Ana C. Messias,Riddhiman Sarkar,Michael Sattler,Don C. Lamb,Matthias J. Feige,Matthias J. Feige,Carlo Camilloni,Carlo Camilloni,Aphrodite Kapurniotu,Bernd Reif +20 more
TL;DR: It is found that the ISM peptide R3‐GI is highly dynamic, can adopt a β‐like structure, and oligomerizes into colloid‐like assemblies in a process that is reminiscent of liquid–liquid phase separation (LLPS), suggesting that such assemblies yield multivalent surfaces for interactions with Aβ40.
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Liquid-liquid phase separation of α-Synuclein: A new mechanistic insight for α-Synuclein aggregation associated with Parkinson's disease pathogenesis.
Semanti Mukherjee,A. Sakunthala,Laxmikant G. Gadhe,Manisha Poudyal,Ajay Singh Sawner,Pradeep Kadu,Samir K. Maji +6 more
TL;DR: In this article , the authors demonstrate the recent development of α-Syn LLPS, the underlying mechanism along with the microscopic events of aberrant phase transition, and further discuss how several intrinsic and extrinsic factors regulate the thermodynamics and kinetics of αSynLLPS and co-LLPS with other proteins, which might explain the pathophysiology of α Syn in various neurodegenerative diseases.
Journal ArticleDOI
Reaction kinetics in open reactors and serial transfers between closed reactors
TL;DR: In this paper, it was shown that the CSTR and serial transfer dynamics are equivalent in the limit where the time interval between the transfers tends to zero proportionally to the ratio of the fractions of fresh to transferred solutions.
References
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PGL-1, a Predicted RNA-Binding Component of Germ Granules, Is Essential for Fertility in C. elegans
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