Journal ArticleDOI
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
Clifford P. Brangwynne,Christian R. Eckmann,David S. Courson,Agata Rybarska,Carsten Hoege,Jöbin Gharakhani,Jöbin Gharakhani,Frank Jülicher,Frank Jülicher,Anthony A. Hyman,Anthony A. Hyman +10 more
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TLDR
It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell.Abstract:
In sexually reproducing organisms, embryos specify germ cells, which ultimately generate sperm and eggs In Caenorhabditis elegans, the first germ cell is established when RNA and protein-rich P granules localize to the posterior of the one-cell embryo Localization of P granules and their physical nature remain poorly understood Here we show that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which we used to estimate their viscosity and surface tension As with other liquids, P granules rapidly dissolved and condensed Localization occurred by a biased increase in P granule condensation at the posterior This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasmread more
Citations
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Receptor tyrosine kinases regulate signal transduction through a liquid-liquid phase separated state
Chi-Chuan Lin,Kin Man Suen,Polly-Anne Jeffrey,Lukasz Wieteska,Jessica A. Lidster,Peng Bao,Alistair Curd,Amy K. Stainthorp,Caroline L Seiler,Hans Koss,Eric A. Miska,Zamal Ahmed,Stephen D. Evans,Carmen Molina-París,John E. Ladbury +14 more
TL;DR: In this article , a subset of phosphorylation-dependent RTK-mediated liquid-liquid phase separation (LLPS) states was investigated. But the authors focused on the formation of phase-separated droplets comprising a ternary complex including the RTK, (FGFR2); the phosphatase, SHP2; and the phospholipase, PLCγ1, which assembles in response to receptor activation.
Journal ArticleDOI
Integration of Data from Liquid-Liquid Phase Separation Databases Highlights Concentration and Dosage Sensitivity of LLPS Drivers.
TL;DR: In this article, a high-confidence dataset of 89 human liquid-liquid phase separation (LLPS) drivers was used to investigate the role of proteins in the LLPS process and found that genes coding for their human LLPS drivers tend to be dosage sensitive.
Journal ArticleDOI
YAP/TAZ phase separation for transcription.
TL;DR: Two recent reports show that both YAP and TAZ form liquid–liquid phase-separated bodies that promote gene transcription by engaging in super-enhancers.
Posted ContentDOI
m6A-binding YTHDF proteins promote stress granule formation by modulating phase separation of stress granule proteins
TL;DR: It is shown that m 6A-modified mRNAs are enriched in SGs, and that m6A-binding YTHDF proteins are critical for SG formation, and both the N-terminal intrinsically disordered region and the C-terminals of the m6a- binding YTH domain are crucial forSG formation.
Journal ArticleDOI
Salt-Induced Liquid-Liquid Phase Separation: Combined Experimental and Theoretical Investigation of Water-Acetonitrile-Salt Mixtures.
TL;DR: In this paper, the authors present results of a systematic investigation of the phase behaviors in water-acetonitrile-salt mixtures using a combination of experiment and theory.
References
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Karen E. Kasza,Amy C. Rowat,Jiayu Liu,Thomas E. Angelini,Clifford P. Brangwynne,Gijsje H. Koenderink,David A. Weitz +6 more
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Journal ArticleDOI
PGL-1, a Predicted RNA-Binding Component of Germ Granules, Is Essential for Fertility in C. elegans
TL;DR: It is shown that a component of germ granules is essential for fertility in C. elegans and that its primary function is in germline proliferation.
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