Journal ArticleDOI
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
Clifford P. Brangwynne,Christian R. Eckmann,David S. Courson,Agata Rybarska,Carsten Hoege,Jöbin Gharakhani,Jöbin Gharakhani,Frank Jülicher,Frank Jülicher,Anthony A. Hyman,Anthony A. Hyman +10 more
Reads0
Chats0
TLDR
It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell.Abstract:
In sexually reproducing organisms, embryos specify germ cells, which ultimately generate sperm and eggs In Caenorhabditis elegans, the first germ cell is established when RNA and protein-rich P granules localize to the posterior of the one-cell embryo Localization of P granules and their physical nature remain poorly understood Here we show that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which we used to estimate their viscosity and surface tension As with other liquids, P granules rapidly dissolved and condensed Localization occurred by a biased increase in P granule condensation at the posterior This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasmread more
Citations
More filters
Journal ArticleDOI
Polyubiquitin chain-induced p62 phase separation drives autophagic cargo segregation
TL;DR: It is reported that p62 forms droplets in vivo which have liquid-like properties such as high sphericity, the ability to undergo fusion, and recovery after photobleaching, and it is proposed that polyubiquitin chain-induced p62 phase separation drives autophagic cargo concentration and segregation.
Journal ArticleDOI
Mechanistic insights into mammalian stress granule dynamics
TL;DR: Panas et al. review the mechanisms that control the dynamic formation and disassembly of RNA stress granules and highlight the importance of knowing the carrier and removal status of canine coronavirus.
Journal ArticleDOI
A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing
Ailin Wang,Alexander E. Conicella,Hermann Broder Schmidt,Erik W. Martin,Shannon N Rhoads,Ashley N. Reeb,Amanda Nourse,Daniel Ramirez Montero,Veronica H Ryan,Rajat Rohatgi,Frank Shewmaker,Mandar T. Naik,Tanja Mittag,Yuna M. Ayala,Nicolas L. Fawzi +14 more
TL;DR: The fusion‐tag solubilized, recombinant form of TDP‐43 full‐length protein developed here will enable future phase separation and in vitro biochemical assays on TDP43 function and interactions that have been hampered in the past by T DP‐43 aggregation.
Journal ArticleDOI
Visualization of dynamics of single endogenous mRNA labeled in live mouse.
Hye Yoon Park,Hyungsik Lim,Young J. Yoon,Antonia Follenzi,Antonia Follenzi,Chiso Nwokafor,Chiso Nwokafor,Melissa Lopez-Jones,Xiuhua Meng,Robert H. Singer +9 more
TL;DR: A transgenic mouse with fluorescently labeled endogenous β-actin mRNA permits single-molecule analysis in live cells and provides insight into its dynamic regulation within the context of the cellular and tissue microenvironment.
Journal ArticleDOI
Polarization of PAR proteins by advective triggering of a pattern-forming system.
Nathan W. Goehring,Philipp Khuc Trong,Justin S. Bois,Debanjan Chowdhury,Ernesto M. Nicola,Anthony A. Hyman,Stephan W. Grill +6 more
TL;DR: Evidence that PAR polarity arises from coupling of advective transport by the flowing cell cortex to a multistable PAR reaction-diffusion system is presented and it is suggested that passive advection transport in an active and flowing material may be a general mechanism for mechanochemical pattern formation in developmental systems.
References
More filters
Journal ArticleDOI
RNA-binding proteins: modular design for efficient function.
TL;DR: These studies have shown how, for many RNA-binding proteins, multiple modules define the fundamental structural unit that is responsible for biological function.
Journal ArticleDOI
The cell as a material.
Karen E. Kasza,Amy C. Rowat,Jiayu Liu,Thomas E. Angelini,Clifford P. Brangwynne,Gijsje H. Koenderink,David A. Weitz +6 more
TL;DR: Reconstituted cytoskeletal protein networks have been shown to mimic many aspects of the mechanical properties of cells, providing new insight into the origin of cellular behavior.
Journal ArticleDOI
Direct visual observation of thermal capillary waves.
TL;DR: It is shown that capillary waves induce the spontaneous breakup of thin liquid films and thus are of key importance in the process of droplet coalescence.
Journal ArticleDOI
Pathway to Totipotency: Lessons from Germ Cells
TL;DR: Common themes emerging from the study of germ cells in vertebrates and invertebrates are described, including transcriptional repression, chromatin remodeling, and an emphasis on posttranscriptional gene regulation that preserve the totipotent genome of germ cell through generations.
Journal ArticleDOI
PGL-1, a Predicted RNA-Binding Component of Germ Granules, Is Essential for Fertility in C. elegans
TL;DR: It is shown that a component of germ granules is essential for fertility in C. elegans and that its primary function is in germline proliferation.
Related Papers (5)
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation
Avinash Patel,Hyun O. Lee,Louise Jawerth,Shovamayee Maharana,Marcus Jahnel,Marco Y. Hein,Stoyno S. Stoynov,Julia Mahamid,Shambaditya Saha,Titus M. Franzmann,Andrej Pozniakovski,Ina Poser,Nicola Maghelli,Loic Royer,Martin Weigert,Eugene W. Myers,Stephan W. Grill,David N. Drechsel,Anthony A. Hyman,Simon Alberti +19 more