Human amniotic fluid stem cell differentiation along smooth muscle lineage
Marco Ghionzoli,Andrea Repele,Laura Sartiani,Giulia Costanzi,Astrid Parenti,Valentina Spinelli,Anna L. David,Massimo Garriboli,Giorgia Totonelli,Jun Tian,Stelios T. Andreadis,Elisabetta Cerbai,Alessandro Mugelli,Antonio Messineo,Agostino Pierro,Simon Eaton,Paolo De Coppi +16 more
TLDR
It is shown here that hAFSCs under selective culture conditions are able to give rise to functional SMCs, for the first time that efficient SMCs can be obtained from human amniotic fluid stem cells.Abstract:
Functional smooth muscle engineering requires isolation and expansion of smooth muscle cells (SMCs), and this process is particularly challenging for visceral smooth muscle tissue where progenitor cells have not been clearly identified. Herein we showed for the first time that efficient SMCs can be obtained from human amniotic fluid stem cells (hAFSCs). Clonal lines were generated from c-kit(+) hAFSCs. Differentiation toward SM lineage (SMhAFSCs) was obtained using a medium conditioned by PDGF-BB and TGF-β1. Molecular assays revealed higher level of α smooth muscle actin (α-SMA), desmin, calponin, and smoothelin in SMhAFSCs when compared to hAFSCs. Ultrastructural analysis demonstrated that SMhAFSCs also presented in the cytoplasm increased intermediate filaments, dense bodies, and glycogen deposits like SMCs. SMhAFSC metabolism evaluated via mass spectrometry showed higher glucose oxidation and an enhanced response to mitogenic stimuli in comparison to hAFSCs. Patch clamp of transduced hAFSCs with lentiviral vectors encoding ZsGreen under the control of the α-SMA promoter was performed demonstrating that SMhAFSCs retained a smooth muscle cell-like electrophysiological fingerprint. Eventually SMhAFSCs contractility was evident both at single cell level and on a collagen gel. In conclusion, we showed here that hAFSCs under selective culture conditions are able to give rise to functional SMCs.read more
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Tissue engineering of urinary bladder and urethra: advances from bench to patients.
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Serum-free spheroid suspension culture maintains mesenchymal stem cell proliferation and differentiation potential.
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Tissue-Engineered Vascular Grafts Created From Human Induced Pluripotent Stem Cells
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TL;DR: Results provide significant insight into the utility of hiPS cells for vascular graft generation and pave the way for creating personalized, patient‐specific vascular grafts for surgical applications, as well as for creating experimental models of vascular development and disease.
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