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Hyperthermophilic Composting Accelerates the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements in Sewage Sludge

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TLDR
It is suggested that hyperthermophilic composting can significantly enhance the removal of ARGs and MGEs and that the mechanisms of ARG and M GE removal can depend on composting temperature.
Abstract
Composting is an efficient way to convert organic waste into fertilizers. However, waste materials often contain large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) that can reduce the efficacy of antibiotic treatments when transmitted to humans. Because conventional composting often fails to remove these compounds, we evaluated if hyperthermophilic composting with elevated temperature is more efficient at removing ARGs and MGEs and explored the underlying mechanisms of ARG removal of the two composting methods. We found that hyperthermophilic composting removed ARGs and MGEs more efficiently than conventional composting (89% and 49%, respectively). Furthermore, the half-lives of ARGs and MGEs were lower in hyperthermophilic compositing compared to conventional composting (67% and 58%, respectively). More-efficient removal of ARGs and MGEs was associated with a higher reduction in bacterial abundance and diversity of potential ARG hosts. Partial least-squares path modeling suggested that reduction of MGEs played a key role in ARG removal in hyperthermophilic composting, while ARG reduction was mainly driven by changes in bacterial community composition under conventional composting. Together these results suggest that hyperthermophilic composting can significantly enhance the removal of ARGs and MGEs and that the mechanisms of ARG and MGE removal can depend on composting temperature.

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antibiotic resistance genes and mobile genetic elements in sewage sludge.
White Rose Research Online URL for this paper:
https://eprints.whiterose.ac.uk/125756/
Version: Accepted Version
Article:
Liao, Hanpeng, Lu, Xiaomei, Rensing, Christopher et al. (7 more authors) (2017)
Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and
mobile genetic elements in sewage sludge. Environmental Science and Technology. 266–
276. ISSN 1520-5851
https://doi.org/10.1021/acs.est.7b04483
eprints@whiterose.ac.uk
https://eprints.whiterose.ac.uk/
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Article
Hyperthermophilic composting accelerates the removal of antibiotic
resistance genes and mobile genetic elements in sewage sludge
Hanpeng Liao, Xiaomei Lu, Christopher Rensing, Ville Petri Friman, Stefan
Geisen, Zhi Chen, Zhen Yu, Zhong Wei, Shungui Zhou, and Yongguan Zhu
Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b04483 • Publication Date (Web): 04 Dec 2017
Downloaded from http://pubs.acs.org on December 4, 2017
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Environmental Science & Technology

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Citations
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Journal ArticleDOI

Hyperthermophilic composting reduces nitrogen loss via inhibiting ammonifiers and enhancing nitrogenous humic substance formation.

TL;DR: It is demonstrated for the first time that hyperthermophilic composting (hTC) was able to mitigate nitrogen loss by 40.9% compared to cTC after 44 days of composting in a full-scale plant and insights into the role of humic substances in nitrogen retention in composting systems are provided.
Journal ArticleDOI

Effects of passivators on antibiotic resistance genes and related mechanisms during composting of copper-enriched pig manure

TL;DR: Overall, all of three passivators can be used to reduce the health risks associated with ARGs in livestock manure, and biochar performed the best at reducing ARGs and MGEs.
Journal ArticleDOI

Antibiotic and metal resistance genes are closely linked with nitrogen-processing functions in municipal solid waste landfills.

TL;DR: Leachates sampled from five representative municipal solid waste landfills in China indicated that more than 85% of sequenced ARGs/HMRGs and nitrogen processing genes, particularly of the denitrification genes, were hosted by the same bacterial species, which belonged to the predominant phylum in leachates.
Journal ArticleDOI

Mobile genetic elements in potential host microorganisms are the key hindrance for the removal of antibiotic resistance genes in industrial-scale composting with municipal solid waste

TL;DR: Investigation of the ARG distributions at different stages in an industrial-scale MSW composting plant found that water-extractable S and pH were two main physicochemical factors in the changes of microbial community and the abundance of ARGs.
References
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Journal ArticleDOI

Search and clustering orders of magnitude faster than BLAST

Robert C. Edgar
- 01 Oct 2010 - 
TL;DR: UCLUST is a new clustering method that exploits USEARCH to assign sequences to clusters and offers several advantages over the widely used program CD-HIT, including higher speed, lower memory use, improved sensitivity, clustering at lower identities and classification of much larger datasets.
Journal ArticleDOI

Metagenomic biomarker discovery and explanation

TL;DR: A new method for metagenomic biomarker discovery is described and validates by way of class comparison, tests of biological consistency and effect size estimation to address the challenge of finding organisms, genes, or pathways that consistently explain the differences between two or more microbial communities.
Journal ArticleDOI

PLS path modeling

TL;DR: PLS path modeling can be used for analyzing multiple tables so as to be related to more classical data analysis methods used in this field and some new improvements are proposed.
Journal ArticleDOI

An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea

TL;DR: A ‘taxonomy to tree’ approach for transferring group names from an existing taxonomy to a tree topology is developed and used to apply the Greengenes, National Center for Biotechnology Information (NCBI) and cyanoDB (Cyanobacteria only) taxonomies to a de novo tree comprising 408 315 sequences.
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Frequently Asked Questions (2)
Q1. What contributions have the authors mentioned in the paper "Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge" ?

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