scispace - formally typeset
Open AccessJournal ArticleDOI

Hyperthermophilic Composting Accelerates the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements in Sewage Sludge

Reads0
Chats0
TLDR
It is suggested that hyperthermophilic composting can significantly enhance the removal of ARGs and MGEs and that the mechanisms of ARG and M GE removal can depend on composting temperature.
Abstract
Composting is an efficient way to convert organic waste into fertilizers. However, waste materials often contain large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) that can reduce the efficacy of antibiotic treatments when transmitted to humans. Because conventional composting often fails to remove these compounds, we evaluated if hyperthermophilic composting with elevated temperature is more efficient at removing ARGs and MGEs and explored the underlying mechanisms of ARG removal of the two composting methods. We found that hyperthermophilic composting removed ARGs and MGEs more efficiently than conventional composting (89% and 49%, respectively). Furthermore, the half-lives of ARGs and MGEs were lower in hyperthermophilic compositing compared to conventional composting (67% and 58%, respectively). More-efficient removal of ARGs and MGEs was associated with a higher reduction in bacterial abundance and diversity of potential ARG hosts. Partial least-squares path modeling suggested that reduction of MGEs played a key role in ARG removal in hyperthermophilic composting, while ARG reduction was mainly driven by changes in bacterial community composition under conventional composting. Together these results suggest that hyperthermophilic composting can significantly enhance the removal of ARGs and MGEs and that the mechanisms of ARG and MGE removal can depend on composting temperature.

read more

Content maybe subject to copyright    Report

This is a repository copy of Hyperthermophilic composting accelerates the removal of
antibiotic resistance genes and mobile genetic elements in sewage sludge.
White Rose Research Online URL for this paper:
https://eprints.whiterose.ac.uk/125756/
Version: Accepted Version
Article:
Liao, Hanpeng, Lu, Xiaomei, Rensing, Christopher et al. (7 more authors) (2017)
Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and
mobile genetic elements in sewage sludge. Environmental Science and Technology. 266–
276. ISSN 1520-5851
https://doi.org/10.1021/acs.est.7b04483
eprints@whiterose.ac.uk
https://eprints.whiterose.ac.uk/
Reuse
Items deposited in White Rose Research Online are protected by copyright, with all rights reserved unless
indicated otherwise. They may be downloaded and/or printed for private study, or other acts as permitted by
national copyright laws. The publisher or other rights holders may allow further reproduction and re-use of
the full text version. This is indicated by the licence information on the White Rose Research Online record
for the item.
Takedown
If you consider content in White Rose Research Online to be in breach of UK law, please notify us by
emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request.

Subscriber access provided by FUJIAN AG & FORESTRY UNIV LIB
Environmental Science & Technology is published by the American Chemical Society.
1155 Sixteenth Street N.W., Washington, DC 20036
Published by American Chemical Society. Copyright © American Chemical Society.
However, no copyright claim is made to original U.S. Government works, or works
produced by employees of any Commonwealth realm Crown government in the course
of their duties.
Article
Hyperthermophilic composting accelerates the removal of antibiotic
resistance genes and mobile genetic elements in sewage sludge
Hanpeng Liao, Xiaomei Lu, Christopher Rensing, Ville Petri Friman, Stefan
Geisen, Zhi Chen, Zhen Yu, Zhong Wei, Shungui Zhou, and Yongguan Zhu
Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b04483 • Publication Date (Web): 04 Dec 2017
Downloaded from http://pubs.acs.org on December 4, 2017
Just Accepted
“Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted
online prior to technical editing, formatting for publication and author proofing. The American Chemical
Society provides “Just Accepted” as a free service to the research community to expedite the
dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts
appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been
fully peer reviewed, but should not be considered the official version of record. They are accessible to all
readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered
to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published
in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just
Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor
changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers
and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors
or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

       
1

2
3





 
4

 !
"
 #
$
 
! 
%
5
6

&'()*+*,'-
7
( - ,' & .( - * /'* 0 "111
8
2
9

3  ,' 4(+*   ( - ,' &
10
.(-*/'*0"1112
11

56*#7#*!81"55/'*!9!9/)2
12

5  : ,(* ;- 3  ,(* # $%11
13
;-2
14
"
- )* +*  3- .<'   -
15
4 - 3  ,(<' (( = :(*
16
0"1$"12
17
$
> '( )* +  8( - # /0 ;& .(
18
/'*;&11?"2
19
%
)* +  /+ ,' -  3  /+ ,' 
20
.(-*(($1@
21
22
-A@ 
23
,A0B@-@(ACD$<"?1<D$?D"1?
24
25
26
27
28
29
30
Page 1 of 29
ACS Paragon Plus Environment
Environmental Science & Technology


31
  ( 7*  (' (7 0@ 7'
32
7(+((E.F-
33
+(E4,F(-(((*+(
34
7-@6(('('
35
 (- 7 '-  *( ( 7 '-
36
   ( ' . - 4, - G- 
37
-* (  .<' +7 7 ( -@ #
38
-*(('-.-4,(*
39
 ('(ED?H - ?H ('*F@  <'
40
 . - 4, 7 7 *( (-  ('
41
( E$%H - "DH ('*F@ 4 ( '  . -
42
4,7(-7-(+(+-(--'*
43
.@I---(
44
4, *-  9*  .<'  *( ( 7
45
. -( 7 * -' +* (  +( (* (
46
-('(@:*(
47
( ( (* (  '  . - 4,-  
48
(.-4,'(--(@
49
50
+-+((.
51
52
53
54
55
56
57
58
59
60
Page 2 of 29
ACS Paragon Plus Environment
Environmental Science & Technology


EG@D@%(+*@%$(F
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
Page 3 of 29
ACS Paragon Plus Environment
Environmental Science & Technology

Citations
More filters
Journal ArticleDOI

Metagenomic biomarker discovery and explanation

TL;DR: In this article, a new method for metagenomic biomarker discovery by way of class comparison, tests of biological consistency and effect size estimation is described and validated, which addresses the challenge of finding organisms, genes, or pathways that consistently explain the differences between two or more microbial communities.
Journal ArticleDOI

Human and veterinary antibiotics during composting of sludge or manure: Global perspectives on persistence, degradation, and resistance genes.

TL;DR: Additional researches are needed, gathering chemical, microbiological and toxicological data to better understand the implied removal mechanisms, the interactions between both components and the environmental matrices and how composting process could be optimized to reduce the discharge of antibiotics and antibiotic resistance genes into the environment.
Journal ArticleDOI

Enhanced in situ biodegradation of microplastics in sewage sludge using hyperthermophilic composting technology

TL;DR: Results reveal the critical role of hyperthermophilic bacteria in MPs biodegradation during hTC, highlighting a promising strategy for sludge-based MPs removal from the real environment.
Journal ArticleDOI

The distinctive microbial community improves composting efficiency in a full-scale hyperthermophilic composting plant.

TL;DR: It is found that HTC running at the hyperthermophilic and thermophilic phases for 21 days, led to higher composting efficiency and techno-economic advantages over TC, and provided theoretical and practical guidance for managing sewage sludge by HTC.
Journal ArticleDOI

Hyperthermophilic composting accelerates the humification process of sewage sludge: Molecular characterization of dissolved organic matter using EEM-PARAFAC and two-dimensional correlation spectroscopy.

TL;DR: Redundancy analysis enabled the relationship between spectral indices and composting parameters to be explained, demonstrating that these indices can be used for assessing the degree of humification.
References
More filters
Journal ArticleDOI

Different analysis techniques for fluorescence excitation-emission matrix spectroscopy to assess compost maturity.

TL;DR: It is demonstrated that fluorescence regional integration (FRI) had a higher correlation coefficient than that of fluorescence intensities and the ratios of peaks, suggesting that FRI technique is more suitable to characterize the maturity of compost than the other two analysis techniques.
Journal ArticleDOI

Sludge bio-drying: Effective to reduce both antibiotic resistance genes and mobile genetic elements

TL;DR: Although changes of bacterial community contributed the most to ARGs profiles, HGT through MGEs should be paid more attention especially in the thermophilic stage of sludge bio-drying.
Journal ArticleDOI

The production of commercial organic amendments and fertilisers by composting of two-phase olive mill waste ("alperujo")

TL;DR: In this article, the technical feasibility of using composting to transform AL into commercial organic amendments and fertilisers was studied. But the main concern for the Spanish olive oil industry is the disposal of the huge amounts (about 5 Mt in the last olive oil campaign, 2010e2011) of the two-phase olive mill waste or “alperujo” (AL) that it produces.
Journal ArticleDOI

Aerobic digestion reduces the quantity of antibiotic resistance genes in residual municipal wastewater solids

TL;DR: It is demonstrated that aerobic digestion can be used to reduce the quantity of ARGs in untreated wastewater solids, but that rates can vary substantially depending on the reactor design (i.e., batch vs. continuous-flow) and the specific ARG.
Journal ArticleDOI

The role of zero valent iron on the fate of tetracycline resistance genes and class 1 integrons during thermophilic anaerobic co-digestion of waste sludge and kitchen waste.

TL;DR: The overall results of this research suggest that thermophilic anaerobic digestion with Fe0 can be a potential alternative technology for the attenuation of tet and intI1 genes in waste sludge.
Related Papers (5)
Frequently Asked Questions (2)
Q1. What contributions have the authors mentioned in the paper "Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge" ?

This is indicated by the licence information on the White Rose Research Online record for the item. 

4@ + 661 ' - 0 @ 0 ! + ! + 06.71 5 E F $@ 662 @ < - >@ @2 4 (9 @ 3@2 ) 9 @2 ) ( 3@ @2 !@< @2 ! .@ @2 4 G7 @2 . ' @ 3@ 663 - + ( - - + ( ( 7 - ( 7 @ 6+! + 664 1 + 7! - 06681 #5 E F 1D$< 1D@ 665 @ ,@ @2 : 9 * @2 @2 4 &V <6 4@2 < @2 ; ' )@ 4@2 - 4@2 + @2 666 * <. #@2 6 4@ :@ 3 ( ( - ( + - 7< ( + @ 0 !