Journal ArticleDOI
ICAT-based comparative proteomic analysis of non-replicating persistent Mycobacterium tuberculosis.
TLDR
Isotope coded affinity tag-based proteomic analysis was used for the determination of the relative expression of large numbers of M. tuberculosis proteins during oxygen self-depletion under controlled conditions in a multi-chambered fermentor to suggest distinct protein expression profiles in NRP-1 and NRPAbout:
This article is published in Tuberculosis.The article was published on 2006-11-01. It has received 57 citations till now. The article focuses on the topics: Mycobacterium tuberculosis.read more
Citations
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Journal ArticleDOI
Low-Oxygen-Recovery Assay for High-Throughput Screening of Compounds against Nonreplicating Mycobacterium tuberculosis
TL;DR: This high-throughput, luminescence-based low-oxygen-recovery assay (LORA) was developed to screen antimicrobial agents against NRP Mycobacterium tuberculosis and is sufficiently robust for use for primary high- throughput screening of compounds against N RP M. tuberculosis.
Journal ArticleDOI
Discovery of virulence factors of pathogenic bacteria
TL;DR: Comparative genomics, transcriptomics, and proteomics have become the popular tools in discovering the virulence factors in bacterial pathogens and combination of these techniques will accelerate the developments of therapeutic drugs and vaccines in combating bacterial diseases.
Journal ArticleDOI
A systematic evaluation of normalization methods in quantitative label-free proteomics
TL;DR: It is found that variance stabilization normalization (Vsn) reduced variation the most between technical replicates in all examined data sets and performed consistently well in the differential expression analysis.
Journal ArticleDOI
Portrait of a Pathogen: The Mycobacterium tuberculosis Proteome In Vivo
TL;DR: The results of this work may provide the basis for rational drug design – identifying numerous Mtb proteins, from essential kinases to products involved in metal regulation and cell wall remodeling, all present throughout the course of infection.
Book ChapterDOI
Physiology of mycobacteria.
Gregory M. Cook,Michael Berney,Susanne Gebhard,Matthias Heinemann,Robert A. Cox,Olga Danilchanka,Michael Niederweis +6 more
TL;DR: This review covers the growth of the mycobacterial cell and how environmental stimuli are sensed by this bacterium and how adaptation to different environments is described from the viewpoint of nutrient acquisition, energy generation, and regulation.
References
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
TL;DR: An approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures based on isotope-coded affinity tags and tandem mass spectrometry is described.
Journal ArticleDOI
Large-scale analysis of the yeast proteome by multidimensional protein identification technology.
TL;DR: MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date, identifying 131 proteins with three or more predicted transmembrane domains which allowed us to map the soluble domains of many of the integral membrane proteins.
Journal ArticleDOI
An Automated Multidimensional Protein Identification Technology for Shotgun Proteomics
TL;DR: An automated method for shotgun proteomics named MudPIT, which combines multidimensional liquid chromatography with electrospray ionization tandem mass spectrometry, improves the overall analysis of proteomes by identifying proteins of all functional and physical classes.
Journal ArticleDOI
Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling.
TL;DR: A model in which M. tuberculosis arrests growth, decreases its respiration rate and is resistant to isoniazid, rifampicin and metronidazole is established, which is generated a model with which to search for agents active against persistent M.culosis.
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