Journal ArticleDOI
Identification and evaluation of two diagnostic markers linked to Fusarium wilt resistance (race 4) in banana (Musa spp.)
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TLDR
The identified sequence-characterized amplified region (SCAR) markers can be applied for a rapid quality control of FOC4-resistant banana plantlets immediately after the in vitro micropropagation stage and will facilitate marker-assisted selection of new banana cultivars resistant to Foc4.Abstract:
Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (FOC4) results in vascular tissue damage and ultimately death of banana (Musa spp.) plants. Somaclonal variants of in vitro micropropagated banana can hamper success in propagation of genotypes resistant to FOC4. Early identification of FOC4 resistance in micropropagated banana plantlets is difficult, however. In this study, we identified sequence-characterized amplified region (SCAR) markers of banana associated with resistance to FOC4. Using pooled DNA from resistant or susceptible genotypes and 500 arbitrary 10-mer oligonucleotide primers, 24 random amplified polymorphic DNA (RAPD) products were identified. Two of these RAPD markers were successfully converted to SCAR markers, called ScaU1001 (GenBank accession number HQ613949) and ScaS0901 (GenBank accession number HQ613950). ScaS0901 and ScaU1001 could be amplified in FOC4-resistant banana genotypes (“Williams 8818-1” and Goldfinger), but not in five tested banana cultivars susceptible to FOC4. The two SCAR markers were then used to identify a somaclonal variant of the genotype “Williams 8818-1”, which lost resistance to FOC4. Hence, the identified SCAR markers can be applied for a rapid quality control of FOC4-resistant banana plantlets immediately after the in vitro micropropagation stage. Furthermore, ScaU1001 and ScaS0901 will facilitate marker-assisted selection of new banana cultivars resistant to FOC4.read more
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Journal ArticleDOI
Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana
TL;DR: The host response, pathogen diversity, current understanding of biochemical and molecular changes that occur during host and pathogen interaction, developing resistant cultivars through mutation, breeding, transgenic and cisgenic approaches have been discussed to understand host defenses against Fusarium oxysporum and to formulate strategies to develop tolerant cultivars.
Journal ArticleDOI
Genomic Prediction in a Multiploid Crop: Genotype by Environment Interaction and Allele Dosage Effects on Predictive Ability in Banana.
Moses Nyine,Brigitte Uwimana,Nicolas Blavet,Eva Hřibová,Helena Vanrespaille,Michael Batte,Violet Akech,Allan Brown,Jim Lorenzen,Rony Swennen,Jaroslav Doležel +10 more
TL;DR: First empirical evidence of genomic prediction in a multi‐ploidy banana population is presented and the high predictive values show the potential of genomic predictions in banana breeding.
Book ChapterDOI
Bananas and Plantains (Musa spp.)
Allan Brown,Robooni Tumuhimbise,Delphine Amah,Brigitte Uwimana,Moses Nyine,Hassan Mduma,David Talengera,D. Karamura,Jerome Kuriba,Rony Swennen +9 more
TL;DR: While the introduction of genomic resources of this perennial long cycling crop promises to hasten the development of improved cultivars, there is a need to maintain vigorous and committed long-term international breeding programs.
Journal ArticleDOI
A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4.
Yuanyuan Wei,Yuanyuan Wei,Yankun Zhao,Dengbo Zhou,Dengfeng Qi,Kai Li,Wen Tang,Yufeng Chen,Tao Jing,Xiaoping Zang,Jianghui Xie,Wei Wang +11 more
TL;DR: In the pot experiment, the crude extracts can significantly improve the banana plant’s resistance to Foc TR4 and Streptomyces sp.
Journal ArticleDOI
The LYSIN MOTIF‐CONTAINING RECEPTOR‐LIKE KINASE 1 protein of banana is required for perception of pathogenic and symbiotic signals
Lu Zhang,Lu Zhang,Liangbin Yuan,Christian Staehelin,Yin Li,Jiuxiao Ruan,Zhenwei Liang,Zhi-Ping Xie,Wei Wang,Jianghui Xie,Shangzhi Huang +10 more
TL;DR: MaLYK1 represents a central molecular switch that controls defense- and symbiosis-related signaling in banana and could functionally replace that of the chitin elicitor receptor kinase 1 in Arabidopsis thaliana and of a rhizobial LCO (Nod factor) receptor (LjNFR1) in L. japonicus.
References
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Journal ArticleDOI
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Journal ArticleDOI
Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce
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TL;DR: Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce, providing information on the molecular basis of RAPD markers.
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