Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium.
Eirwen Morgan,June Campbell,Sonya C. Rowe,Jennie Bispham,Mark P. Stevens,Alison J. Bowen,Paul A. Barrow,Duncan J. Maskell,Timothy S. Wallis +8 more
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TLDR
It is concluded that S. Typhimurium uses different strategies to colonize calves and chicks, which has major implications for vaccine design.Abstract:
The severity of infections caused by Salmonella enterica serovar Typhimurium varies depending on the host species. Numerous virulence genes have been identified in S. Typhimurium, largely from studies in mice, but their roles in infections of other species remain unclear. In the most comprehensive survey of its kind, through the use of signature-tagged mutagenesis of S. Typhimurium we have identified mutants that were unable to colonize calf intestines, mutants unable to colonize chick intestines and mutants unable to colonize both species. The type three secretion systems encoded on Salmonella pathogenicity islands (SPIs) 1 and 2 were required for efficient colonization of cattle. However, disruption of these secretion systems only caused a minor defect in S. Typhimurium colonization of chicks. Transposon insertions in SPI-4 compromised S. Typhimurium colonization of cattle, but not chicks. This is the first data confirming a role for SPI-4 in pathogenesis. We have also been able to ascribe a role in colonization for cell surface polysaccharides, cell envelope proteins, and many 'housekeeping' genes and genes of unknown function. We conclude that S. Typhimurium uses different strategies to colonize calves and chicks. This has major implications for vaccine design.read more
Citations
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Salmonella enterica Serovar Typhimurium Skills To Succeed in the Host: Virulence and Regulation
Anna Fàbrega,Jordi Vila +1 more
TL;DR: This review focuses primarily on the virulence armamentarium of this pathogen and the extremely complicated regulatory network controlling its success.
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Salmonella biofilms: An overview on occurrence, structure, regulation and eradication
TL;DR: Insight into the pathogen's complex biofilm process will eventually lead to further unraveling of its intricacies and more efficient strategies to combat Salmonella biofilms, as well as the potential of combination therapy.
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Bap: a family of surface proteins involved in biofilm formation.
Iñigo Lasa,José R. Penadés +1 more
TL;DR: This review summarizes recent studies that have identified and assigned roles to Bap-related proteins in biofilm biology and virulence.
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Genome-wide screen for Salmonella genes required for long-term systemic infection of the mouse.
Trevor D. Lawley,Kaman Chan,Lucinda J. Thompson,Charles C. Kim,Gregory R. Govoni,Denise M. Monack +5 more
TL;DR: Data indicate that Salmonella long-term systemic infection in the mouse requires a diverse repertoire of virulence factors, and a progressive selection against serovar Typhimurium mutants based upon the duration of the infection is observed, suggesting that different classes of genes may be required at distinct stages of infection.
Journal ArticleDOI
BapA, a large secreted protein required for biofilm formation and host colonization of Salmonella enterica serovar Enteritidis
Cristina Latasa,Agnès Roux,Alejandro Toledo-Arana,Jean-Marc Ghigo,Carlos Gamazo,José R. Penadés,Iñigo Lasa +6 more
TL;DR: Evidence that overproduction of curli fimbriae and not cellulose can compensate for the biofilm deficiency of a bapA mutant strain is provided, demonstrating that BapA contributes both to biofilm formation and invasion through the regular Salmonella infection route.
References
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