Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692.
Silindile Maphosa,Lucy N. Moleleki +1 more
- Vol. 9, Iss: 9, pp 1918
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TLDR
Pectobacterium brasiliense (Pbr) 1692 is an aggressive phytopathogen affecting a broad host range of crops and ornamental plants, including potatoes.Abstract:
Pectobacterium brasiliense (Pbr) 1692 is an aggressive phytopathogen affecting a broad host range of crops and ornamental plants, including potatoes. Previous research on animal pathogens, and a few plant pathogens, revealed that Outer Membrane Vesicles (OMVs) are part of Gram-negative bacteria's (GNB) adaptive toolkit. For this reason, OMV production and subsequent release from bacteria is a conserved process. Therefore, we hypothesized that OMVs might transport proteins that play a critical role in causing soft rot disease and in the survival and fitness of Pbr1692. Here, we show that the potato pathogen, Pbr1692, releases OMVs of various morphologies in Luria Bertani media at 31 °C. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) confirmed the production of OMVs by Pbr1692 cells. Transmission Electron Microscopy showed that these exist as chain-, single-, and double-membrane morphologies. Mass spectrometry followed by Gene Ontology, Clusters of Orthologous Groups, Virulence Factor, CAZymes, Antibiotic Resistance Ontology, and Bastion6 T6SE annotations identified 129 OMV-associated proteins with diverse annotated roles, including antibiotic stress response, virulence, and competition. Pbr1692 OMVs contributed to virulence in potato tubers and elicited a hypersensitive response in Nicotiana benthamiana leaves. Furthermore, Pbr1692 OMVs demonstrated antibacterial activity against Dickeya dadantii.read more
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Membrane Vesicles of Pectobacterium as an Effective Protein Secretion System
Joanna Jonca,Malgorzata Waleron,Paulina Czaplewska,Aleksandra E. Bogucka,Aleksandra Steć,Szymon Dziomba,Jacek Jasiecki,Michał Rychłowski,Krzysztof Waleron +8 more
TL;DR: In this article, the role of Membrane Vesicles (MVs) in the virulence of Pectobacterium is investigated and it is shown that MVs play an important role in host-pathogen interactions and niche competition with other bacteria.
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