Journal ArticleDOI
Liposome−Cell Interactions in Vitro: Effect of Liposome Surface Charge on the Binding and Endocytosis of Conventional and Sterically Stabilized Liposomes†
TLDR
In this paper, the effect of liposome surfacency on cellular uptake is examined, which is generally believed to be mediated by adsorption of the liposomes onto the cell surface and subsequent endocytosis.Abstract:
The cellular uptake of liposomes is generally believed to be mediated by adsorption of liposomes onto the cell surface and subsequent endocytosis. This report examines the effect of liposome surfac...read more
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Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications
Ajay Kumar Gupta,Mona Gupta +1 more
TL;DR: This review discusses the synthetic chemistry, fluid stabilization and surface modification of superparamagnetic iron oxide nanoparticles, as well as their use for above biomedical applications.
Journal ArticleDOI
Principles of nanoparticle design for overcoming biological barriers to drug delivery
TL;DR: By successively addressing each of the biological barriers that a particle encounters upon intravenous administration, innovative design features can be rationally incorporated that will create a new generation of nanotherapeutics, realizing a paradigmatic shift in nanoparticle-based drug delivery.
Journal ArticleDOI
The effect of particle design on cellular internalization pathways
Stephanie E. A. Gratton,Patricia A. Ropp,Patrick D. Pohlhaus,J. Christopher Luft,Victoria J. Madden,Mary E. Napier,Joseph M. DeSimone +6 more
TL;DR: These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 μm by using several different mechanisms of endocytosis, and it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates.
Journal ArticleDOI
The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles.
TL;DR: Findings on the role of surface charge on cytotoxicity in general, action on specific cellular targets, modes of toxic action, cellular uptake, and intracellular localization of NPs are summarized.
Journal ArticleDOI
Liposomes as nanomedical devices
TL;DR: This review briefly analyze how the efficacy of liposomes depends on the nature of their components and their size, surface charge, and lipidic organization, and describes some strategies developed to overcome limitations of the “first-generation” liposome-based drugs on the market and in clinical trials.
References
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Journal ArticleDOI
Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule
TL;DR: The ability of fibronectin to bind cells can be accounted for by the tetrapeptide L-arginyl-glycyl- L-aspartyl-L-serine, a sequence which is part of the cell attachment domain of fibronsectin and present in at least five other proteins.
Journal ArticleDOI
Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes
TL;DR: The PEG‐PE's activity to prolong the circulation time of liposomes is greater than that of the ganglioside GM1, awell‐described glycolipid with this activity.
Journal ArticleDOI
Amino acid sequence of the human fibronectin receptor.
TL;DR: The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported and suggests a structure for the receptor in which the hydrophobic segments serve as transmembrane domains anchoring each subunit to the membrane and dividing each into a large ectodomain and a short cytoplasmic domain.
Journal ArticleDOI
Sterically stabilized anti-her2 immunoliposomes : design and targeting to human breast cancer cells in vitro
Dmitri B. Kirpotin,John W. Park,Keelung Hong,Samuel Zalipsky,Wen-Lu Li,Paul Carter,Christopher C. Benz,Demetrios Papahadjopoulos +7 more
TL;DR: Cell binding and internalization of anti-HER2 immunoliposomes increased at higher surface density of conjugated Fab' fragments, reaching plateaus at approximately 40 Fab'/liposome for binding and approximately 10-15 Fab'/ Liposomes for internalization, which were used to optimize in vivo preclinical studies ofAnti- HER2 SL loaded with antineoplastic drugs.
Journal ArticleDOI
Association of blood proteins with large unilamellar liposomes in vivo. Relation to circulation lifetimes.
TL;DR: The protein binding ability (PB; g of protein/mol of lipid) of the liposomes was quantitated and related to the circulation half-life (tau 1/2).
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