Mechanistic insights into mammalian stress granule dynamics
TLDR
Panas et al. review the mechanisms that control the dynamic formation and disassembly of RNA stress granules and highlight the importance of knowing the carrier and removal status of canine coronavirus.Abstract:
The accumulation of stalled translation preinitiation complexes (PICs) mediates the condensation of stress granules (SGs). Interactions between prion-related domains and intrinsically disordered protein regions found in SG-nucleating proteins promote the condensation of ribonucleoproteins into SGs. We propose that PIC components, especially 40S ribosomes and mRNA, recruit nucleators that trigger SG condensation. With resolution of stress, translation reinitiation reverses this process and SGs disassemble. By cooperatively modulating the assembly and disassembly of SGs, ribonucleoprotein condensation can influence the survival and recovery of cells exposed to unfavorable environmental conditions.read more
Citations
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G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules
Peiguo Yang,Cécile Mathieu,Cécile Mathieu,Regina-Maria Kolaitis,Peipei Zhang,James Messing,James Messing,Ugur Yurtsever,Ugur Yurtsever,Zemin Yang,Jinjun Wu,Yuxin Li,Qingfei Pan,Jiyang Yu,Erik W. Martin,Tanja Mittag,Hong Joo Kim,J. Paul Taylor,J. Paul Taylor +18 more
TL;DR: It is shown that SGs assemble through liquid-liquid phase separation (LLPS) arising from interactions distributed unevenly across a core protein-RNA interaction network, and that interplay between three distinct intrinsically disordered regions (IDRs) in G3BP1 regulates its intrinsic propensity for LLPS.
Journal ArticleDOI
The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules
Anthony Khong,Anthony Khong,Tyler Matheny,Saumya Jain,Sarah F. Mitchell,Joshua Wheeler,Roy Parker,Roy Parker +7 more
TL;DR: The results suggest that stress granules may not represent a specific biological program of messenger ribonucleoprotein (mRNP) assembly, but instead form by condensation of nontranslating mRNPs in proportion to their length and lack of association with ribosomes.
Journal ArticleDOI
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation
Jordina Guillén-Boixet,Andrii Kopach,Alex S. Holehouse,Sina Wittmann,Marcus Jahnel,Marcus Jahnel,Raimund Schlüßler,Kyoohyun Kim,Irmela R.E.A. Trussina,Jie Wang,Daniel Mateju,Ina Poser,Shovamayee Maharana,Martine Ruer-Gruß,Doris Richter,Xiaojie Zhang,Young-Tae Chang,Jochen Guck,Alf Honigmann,Julia Mahamid,Anthony A. Hyman,Rohit V. Pappu,Simon Alberti,Simon Alberti,Titus M. Franzmann +24 more
TL;DR: It is proposed that condensation coupled to conformational rearrangements and heterotypic multivalent interactions may be a general principle underlying RNP granule assembly.
Journal ArticleDOI
TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics
Ian R. A. Mackenzie,Alexandra M. Nicholson,Mohona Sarkar,James Messing,Maria D. Purice,Cyril Pottier,Kavya Annu,Matt Baker,Ralph B. Perkerson,Aishe Kurti,Billie J. Matchett,Tanja Mittag,Jamshid Temirov,Ging-Yuek Robin Hsiung,Charles Krieger,Charles Krieger,Melissa E. Murray,Masato Kato,John D. Fryer,Leonard Petrucelli,Lorne Zinman,Sandra Weintraub,M.-Marsel Mesulam,Julia Keith,Sasha Zivkovic,Veronica Hirsch-Reinshagen,Raymond P. Roos,Stephan Züchner,Neill R. Graff-Radford,Ronald C. Petersen,Richard J. Caselli,Zbigniew K. Wszolek,Elizabeth Finger,Carol F. Lippa,Carol F. Lippa,David Lacomis,Heather Stewart,Dennis W. Dickson,Hong Joo Kim,Ekaterina Rogaeva,Eileen H. Bigio,Kevin B. Boylan,J. Paul Taylor,Rosa Rademakers +43 more
TL;DR: The identification of TIA1 mutations in ALS/FTD reinforces the importance of RNA metabolism and SG dynamics in ALS-FTD pathogenesis.
Journal ArticleDOI
Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing.
Simon Alberti,Anthony A. Hyman +1 more
TL;DR: A review of the role of biomolecular condensates in ageing and disease can be found in this paper, where the authors discuss how cellular stress, ageing-related loss of homeostasis and a decline in protein quality control may contribute to the formation of aberrant, disease-causing condensate.
References
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