Journal ArticleDOI
Megapixel digital PCR
Kevin A. Heyries,Carolina Tropini,Carolina Tropini,Michael VanInsberghe,C. Doolin,Oleh Petriv,Anupam Singhal,Kaston Leung,Curtis B. Hughesman,Carl L. Hansen +9 more
TLDR
A microfluidic 'megapixel' digital PCR device that uses surface tension–based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactor cm−2 is presented.Abstract:
We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.read more
Citations
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Journal ArticleDOI
Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification
Leonardo B. Pinheiro,Victoria A. Coleman,Christopher Hindson,Jan Herrmann,Benjamin J. Hindson,Somanath Bhat,Kerry R. Emslie +6 more
TL;DR: Analysis of simplex and duplex assays targeting two distinct loci in the Lambda DNA genome using the ddPCR platform agreed, within their expanded uncertainties, with values obtained using a lower density microfluidic chamber based digital PCR (cdPCR).
Journal ArticleDOI
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
Jim F. Huggett,Carole A. Foy,Vladimir Benes,Kerry R. Emslie,Jeremy A. Garson,Ross J. Haynes,Jan Hellemans,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Jo Vandesompele,Carl T. Wittwer,Stephen A. Bustin +14 more
TL;DR: This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation and presents the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines.
Journal ArticleDOI
dPCR: A Technology Review.
TL;DR: This review compares the fundamental concepts behind the quantification of nucleic acids by dPCR and quantitative real-time PCR and examines how isothermal amplification could be an alternative to PCR in digital assays.
Journal ArticleDOI
Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering
Shana O. Kelley,Chad A. Mirkin,David R. Walt,Rustem F. Ismagilov,Mehmet Toner,Edward H. Sargent +5 more
TL;DR: Recent strategies that combine nano- and microscale materials and devices to produce large improvements in detection sensitivity, speed and accuracy, allowing previously undetectable biomarkers to be identified in clinical samples are reviewed.
Journal ArticleDOI
Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
Christofer Toumazou,Leila Shepherd,Samuel Reed,Ginny I Chen,Alpesh Patel,David M Garner,Chan-Ju A Wang,Chung-Pei Ou,Krishna Amin-Desai,Panteleimon Athanasiou,Hua Bai,Ines M Q Brizido,Benjamin Caldwell,Daniel Coomber-Alford,Pantelis Georgiou,Karen S Jordan,John C Joyce,Maurizio La Mura,Daniel Morley,Sreekala Sathyavruthan,Sara Temelso,Risha E Thomas,Linglan Zhang +22 more
TL;DR: This work has developed an integrated chip for real-time amplification and detection of nucleic acid using pH-sensing complementary metal-oxide semiconductor (CMOS) technology and evaluated the platform using two amplification strategies: PCR and isothermal amplification.
References
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