MEROPS: the peptidase database
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TLDR
The MEROPS database has added an analysis tool to the relevant species pages to show significant gains and losses of peptidase genes relative to related species, and has collected over 39 000 known cleavage sites in proteins, peptides and synthetic substrates.Abstract:
Peptidases (proteolytic enzymes) are of great relevance to biology, medicine and biotechnology. This practical importance creates a need for an integrated source of information about them, and also about their natural inhibitors. The MEROPS database (http://merops.sanger.ac.uk) aims to fill this need. The organizational principle of the database is a hierarchical classification in which homologous sets of the proteins of interest are grouped in families and the homologous families are grouped in clans. Each peptidase, family and clan has a unique identifier. The database has recently been expanded to include the protein inhibitors of peptidases, and these are classified in much the same way as the peptidases. Forms of information recently added include new links to other databases, summary alignments for peptidase clans, displays to show the distribution of peptidases and inhibitors among organisms, substrate cleavage sites and indexes for expressed sequence tag libraries containing peptidases. A new way of making hyperlinks to the database has been devised and a BlastP search of our library of peptidase and inhibitor sequences has been added.read more
Citations
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Proteases, cystic fibrosis and the epithelial sodium channel (ENaC).
TL;DR: The regulation of proteases is focused on and specifically those proteases found in human airways are discussed, including those of the most prevalent bacterial pathogen found in cystic fibrosis, Pseudomonas aeruginosa.
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The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis
Jorg Brunner,Floyd R.A. Wittink,Martijs J. Jonker,Mark de Jong,Timo M. Breit,Marja L. Laine,Johannes J. de Soet,Wim Crielaard +7 more
TL;DR: Comparing highly virulent strains with less virulence strains indicates that hmuS, a putative CobN/Mg chelatase involved in heme uptake, may be a more relevant virulence determinant than previously expected.
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Overview of transcriptomic analysis of all human proteases, non-proteolytic homologs and inhibitors: Organ, tissue and ovarian cancer cell line expression profiling of the human protease degradome by the CLIP-CHIP™ DNA microarray
Reinhild Kappelhoff,Xose S. Puente,Claire H. Wilson,Arun Seth,Carlos López-Otín,Christopher M. Overall +5 more
TL;DR: The CLIP-CHIP™, a custom microarray based on a 70-mer oligonucleotide platform, was developed to specifically profile the transcripts of the entire repertoire of 473 active human proteases, 156 protease inhibitors and 92 non-proteolytically active homologs known at the design date using one specific 70-Mer oligon nucleotide per transcript.
Journal ArticleDOI
Expansion and functional diversification of a leucyl aminopeptidase family that encodes the major protein constituents of Drosophila sperm
TL;DR: Comparative genomic and phylogenetic analyses revealed the dramatic expansion of the S-LAP gene family during Drosophila evolution and copy number heterogeneity in the genomes of related insects.
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Control of Entamoeba histolytica Adherence Involves Metallosurface Protease 1, an M8 Family Surface Metalloprotease with Homology to Leishmanolysin
TL;DR: E. histolytica metallosurface protease 1 was shown to be a surface metalloprotease involved in regulation of amebic adherence, with additional effects on cell motility, cell monolayer destruction, and phagocytosis.
References
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