MEROPS: the peptidase database
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TLDR
The MEROPS database has added an analysis tool to the relevant species pages to show significant gains and losses of peptidase genes relative to related species, and has collected over 39 000 known cleavage sites in proteins, peptides and synthetic substrates.Abstract:
Peptidases (proteolytic enzymes) are of great relevance to biology, medicine and biotechnology. This practical importance creates a need for an integrated source of information about them, and also about their natural inhibitors. The MEROPS database (http://merops.sanger.ac.uk) aims to fill this need. The organizational principle of the database is a hierarchical classification in which homologous sets of the proteins of interest are grouped in families and the homologous families are grouped in clans. Each peptidase, family and clan has a unique identifier. The database has recently been expanded to include the protein inhibitors of peptidases, and these are classified in much the same way as the peptidases. Forms of information recently added include new links to other databases, summary alignments for peptidase clans, displays to show the distribution of peptidases and inhibitors among organisms, substrate cleavage sites and indexes for expressed sequence tag libraries containing peptidases. A new way of making hyperlinks to the database has been devised and a BlastP search of our library of peptidase and inhibitor sequences has been added.read more
Citations
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Journal ArticleDOI
Computational identification of uncharacterized cruzain binding sites.
TL;DR: Molecular dynamics simulations and a sequence alignment of a non-redundant, unbiased set of peptidase C1 family members are used to identify uncharacterized cruzain binding sites and the two sites identified may serve as targets for future pharmacological intervention.
Journal ArticleDOI
Linker and/or transmembrane regions of influenza A/Group-1, A/Group-2, and type B virus hemagglutinins are packed differently within trimers
Larisa V. Kordyukova,Marina V. Serebryakova,Anton A. Polyansky,Kropotkina Ea,Andrei V. Alexeevski,Michael Veit,Roman G. Efremov,Irina Yu. Filippova,Lyudmila A. Baratova +8 more
TL;DR: It is proposed that the A/Group-2 hemagglutinin linker and/or transmembrane regions are more tightly associated within trimers than type A/ group-1 and particularly type B ones.
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The Pratylenchus penetrans Transcriptome as a Source for the Development of Alternative Control Strategies: Mining for Putative Genes Involved in Parasitism and Evaluation of in planta RNAi
Paulo Vieira,Paulo Vieira,Sebastian Eves-van den Akker,Ruchi Verma,Sarah Wantoch,Jonathan D. Eisenback,Kathryn Kamo +6 more
TL;DR: Light is shed on the transcriptional changes that accompany plant infection by P. penetrans, and will aid in identifying potential gene targets for selection and use to design effective control strategies against root lesion nematodes.
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Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid.
TL;DR: It is shown that ANCE, which shares many enzymatic properties with mammalian ACE, is also a product of the male accessory gland of D. melanogaster and is expressed in the secondary cells and is associated with the electron dense granule within the large vesicles of these cells.
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Type I cystatin (stefin) is a major component of Fasciola gigantica excretion/secretion product.
TL;DR: Protective functions of FgStefin-1 are suggested, regulating intracellular cysteine protease activity, and possibly protection against extracellular proteolytic damage to the parasite's intestinal and tegumental surface proteins.
References
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