MEROPS: the peptidase database
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TLDR
The MEROPS database has added an analysis tool to the relevant species pages to show significant gains and losses of peptidase genes relative to related species, and has collected over 39 000 known cleavage sites in proteins, peptides and synthetic substrates.Abstract:
Peptidases (proteolytic enzymes) are of great relevance to biology, medicine and biotechnology. This practical importance creates a need for an integrated source of information about them, and also about their natural inhibitors. The MEROPS database (http://merops.sanger.ac.uk) aims to fill this need. The organizational principle of the database is a hierarchical classification in which homologous sets of the proteins of interest are grouped in families and the homologous families are grouped in clans. Each peptidase, family and clan has a unique identifier. The database has recently been expanded to include the protein inhibitors of peptidases, and these are classified in much the same way as the peptidases. Forms of information recently added include new links to other databases, summary alignments for peptidase clans, displays to show the distribution of peptidases and inhibitors among organisms, substrate cleavage sites and indexes for expressed sequence tag libraries containing peptidases. A new way of making hyperlinks to the database has been devised and a BlastP search of our library of peptidase and inhibitor sequences has been added.read more
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A Parasite Cysteine Protease Is Key to Host Protein Degradation and Iron Acquisition
Theresa C. O’Brien,Zachary B. Mackey,Richard D. Fetter,Youngchool Choe,Anthony J. O’Donoghue,Min Zhou,Charles S. Craik,Conor R. Caffrey,James H. McKerrow +8 more
TL;DR: Western blot and microscopic analysis indicate that deficiency in tbcatB results in accumulation of both host and parasite proteins, including the lysosomal marker p67, which is necessary for iron acquisition for parasitism.
Journal ArticleDOI
Isolation and characterization of two serine proteases from metagenomic libraries of the Gobi and Death Valley deserts.
TL;DR: 17 potential protease-producing clones from two metagenomic libraries derived from samples of surface sand from the Gobi and Death Valley deserts are identified and two serine proteases appear to belong to the subtilisin (S8A) family and displayed several unique biochemical properties.
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Microbial Keratinases: Enzymes with Promising Biotechnological Applications.
Beti Vidmar,Maša Vodovnik +1 more
TL;DR: A comprehensive review covering molecular properties and characteristics of keratinases, their classification, traditional and novel approaches in discovery of novel enzymes, production, characterization, improvement and biotechnological applications is presented.
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Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape
TL;DR: The genome-wide identification, evolutionary and expression analyses of grape AP genes provide a framework for future analysis of AP genes in defining their roles during stress response, and integrated synteny and phylogenetic analyses provide novel insight into the functions of less well-studied genes using information from their better understood orthologs.
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Distinctive modulation of inflammatory and metabolic parameters in relation to zinc nutritional status in adult overweight/obese subjects.
Laura Costarelli,Elisa Muti,Marco Malavolta,Catia Cipriano,Robertina Giacconi,Silvia Tesei,Francesco Piacenza,Sara Pierpaoli,Nazzarena Gasparini,Emanuela Faloia,Giacomo Tirabassi,Marco Boscaro,Angela Polito,Beatrice Mauro,Francesca Maiani,Anna Raguzzini,Fiorella Marcellini,Cinzia Giuli,Roberta Papa,Monica Emanuelli,Fabrizia Lattanzio,Eugenio Mocchegiani +21 more
TL;DR: A possible zinc supplementation in obese subjects with a deeper inflammatory status and more altered zinc profile may be suggested in order to limit or reduce the inflammation, taking also into account that zinc supplementation normalizes "inflammaging" as well as zinc profile leading to a correct intra- and extracellular zinc homeostasis.
References
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