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Open AccessJournal ArticleDOI

Metabolomics of Mycobacterium tuberculosis Reveals Compartmentalized Co-Catabolism of Carbon Substrates

Luiz Pedro S. de Carvalho, +5 more
- 29 Oct 2010 - 
- Vol. 17, Iss: 10, pp 1122-1131
TLDR
Surprisingly, untargeted metabolite profiling of Mtb growing on ¹³C-labeled carbon substrates revealed that Mtb could catabolize multiple carbon sources simultaneously to achieve enhanced monophasic growth.
About: 
This article is published in Chemistry & Biology.The article was published on 2010-10-29 and is currently open access. It has received 315 citations till now. The article focuses on the topics: Catabolite repression.

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Citations
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Journal ArticleDOI

Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis.

Michael A. DeJesus, +12 more
- 08 Mar 2017 - 
TL;DR: This work provides an authoritative catalog of essential regions of the M. tuberculosis genome and a statistical framework for applying saturating mutagenesis to other bacteria.
Journal ArticleDOI

Cholesterol catabolism by Mycobacterium tuberculosis requires transcriptional and metabolic adaptations.

Jennifer E. Griffin, +8 more
- 24 Feb 2012 - 
TL;DR: It is shown that both Rv1129c and the MCC enzymes are required for intracellular growth in macrophages and that the growth defect of MCC mutants is largely attributable to the degradation of host-derived cholesterol.
Journal ArticleDOI

Isocitrate lyase mediates broad antibiotic tolerance in Mycobacterium tuberculosis

Madhumitha Nandakumar, +2 more
- 30 Jun 2014 - 
TL;DR: In this paper, the authors apply metabolomic profiling to discover a common set of metabolic changes associated with the activities of three clinically used tuberculosis drugs, isoniazid, rifampicin and streptomycin.
Journal ArticleDOI

Multifunctional essentiality of succinate metabolism in adaptation to hypoxia in Mycobacterium tuberculosis

Hyungjin Eoh, +1 more
- 16 Apr 2013 - 
TL;DR: It is shown that, as M. tuberculosis adapts to hypoxia, it slows and remodels its tricarboxylic acid cycle to increase production of succinate, which is used to flexibly sustain membrane potential, ATP synthesis, and anaplerosis, in response to varying degrees of O2 limitation and the presence of the alternate electron acceptor nitrate.
Journal ArticleDOI

Novel inhibitors of cholesterol degradation in Mycobacterium tuberculosis reveal how the bacterium's metabolism is constrained by the intracellular environment.

Brian C. VanderVen, +12 more
- 12 Feb 2015 - 
TL;DR: Mycobacterium tuberculosis (Mtb) relies on a specialized set of metabolic pathways to support growth in macrophages and is subject to a unique form of metabolic constraint induced by the presence of cholesterol, which implicate cyclic-AMP (cAMP) in regulating cholesterol utilization in Mtb.
References
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Journal ArticleDOI

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford
- 07 May 1976 - 
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book

Principles and Practice of Infectious Diseases

Gerald L. Mandell, +2 more
TL;DR: This updated and expanded edition now offers 297 chapters that cover the basic principles of diagnosis and management, major clinical syndromes, all important pathogenic microbes and the diseases they cause, plus a number of specialised topics useful to the practitioner.
Journal ArticleDOI

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

Stewart T. Cole, +41 more
- 11 Jun 1998 - 
TL;DR: The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve the understanding of the biology of this slow-growing pathogen and to help the conception of new prophylactic and therapeutic interventions.
Journal ArticleDOI

Mass spectrometry-based metabolomics.

Katja Dettmer, +2 more
- 01 Jan 2007 - 
TL;DR: This review presents an overview of the dynamically developing field of mass spectrometry-based metabolomics, a technique that analyzes all detectable analytes in a given sample with subsequent classification of samples and identification of differentially expressed metabolites, which define the sample classes.
Journal ArticleDOI

Carbon catabolite repression in bacteria: many ways to make the most out of nutrients

Boris Görke, +1 more
- 01 Aug 2008 - 
TL;DR: The most recent findings on the different mechanisms that have evolved to allow bacteria to use carbon sources in a hierarchical manner are discussed.
Related Papers (5)

Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection

Joeli Marrero, +4 more
- 25 May 2010 - 

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

Stewart T. Cole, +41 more
- 11 Jun 1998 - 

Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence

Ernesto J. Muñoz-Elías, +1 more
- 15 May 2005 - 

Mycobacterial persistence requires the utilization of host cholesterol

Amit K. Pandey, +1 more
- 18 Mar 2008 - 

Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase

John D. McKinney, +17 more
- 17 Aug 2000 -