Methylation as a Crucial Step in Plant microRNA Biogenesis
Bin Yu,Zhiyong Yang,Junjie Li,Svetlana Minakhina,Maocheng Yang,Richard W. Padgett,Ruth Steward,Xuemei Chen +7 more
TLDR
It is shown that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide, a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.Abstract:
Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2'-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.read more
Citations
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Biogenesis of small RNAs in animals.
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Small silencing RNAs: an expanding universe
TL;DR: Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs.
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microRNA-Directed Phasing during Trans-Acting siRNA Biogenesis in Plants
TL;DR: Data support a model in which miRNA-guided formation of a 5' or 3' terminus within pre-ta-siRNA transcripts, followed by RDR6-dependent formation of dsRNA and Dicer-like processing, yields phased ta-siRNAs that negatively regulate other genes.
References
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Zhixin Xie,Lisa K. Johansen,Adam M. Gustafson,Kristin D. Kasschau,Andrew D Lellis,Daniel Zilberman,Steven E. Jacobsen,James C. Carrington +7 more
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CARPEL FACTORY, a Dicer Homolog, and HEN1, a Novel Protein, Act in microRNA Metabolism in Arabidopsis thaliana
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