miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
Citations
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Associations of miRNA polymorphisms and female physiological characteristics with breast cancer risk in Chinese population
Ming Zhang,Mingjuan Jin,Ying Yu,Suzhan Zhang,Yulian Wu,He Liu,Banglin Chen,Q. Li,X. Ma,Kun Chen +9 more
TL;DR: It is concluded that menarche at less than 16 years old increased breast cancer risk, while the genetic variants in miR-196-a-2 might decrease the risk.
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MicroRNA-30a-3p inhibits tumor proliferation, invasiveness and metastasis and is downregulated in hepatocellular carcinoma.
Wei Wang,Huapeng Lin,Lin Zhou,Q. Zhu,Shun-liang Gao,Haiyang Xie,Zhikun Liu,Z. Xu,Juying Wei,Xiao-Jun Huang,Shufa Zheng +10 more
TL;DR: The data suggest that miR-30a-3p is downregulated in HCC and acts as a tumor suppressor in vitro, and Regulation of vimentin, E-cadherin and MMP3 by miR,30a,3p suggests a useful therapeutic strategy for tumors with reduced miR+3p expression.
Journal ArticleDOI
MicroRNA-133a Suppresses Multiple Oncogenic Membrane Receptors and Cell Invasion in Non-Small Cell Lung Carcinoma
Lu Kai Wang,Tzu Hung Hsiao,Tse-Ming Hong,Hsuan-Yu Chen,Shih Han Kao,Wen Lung Wang,Sung-Liang Yu,Ching Wen Lin,Pan-Chyr Yang +8 more
TL;DR: The tumor suppressor role of miR-133a in lung cancer outcome prognosis is identified, and it is demonstrated that it targets several membrane receptors, which generally produce an activating signaling network during the progression of lung cancer.
Journal ArticleDOI
MicroRNA-196a Is a Putative Diagnostic Biomarker and Therapeutic Target for Laryngeal Cancer
Koichiro Saito,Koji Inagaki,Takahiro Kamimoto,Yoko Ito,Toshiaki Sugita,Satoko Nakajo,Akira Hirasawa,Arifumi Iwamaru,Takashi Ishikura,Hideki Hanaoka,Keisuke Okubo,Tokio Onozaki,Takeru Zama +12 more
TL;DR: This study provided the possibilities that miR-196a might be very useful in diagnosing and treating laryngeal cancer and validated its status as the most promising cancer biomarker as validated by qRT-PCR analyses on additional 84 tissue samples.
Journal ArticleDOI
Circulating microRNA expressions in colorectal cancer as predictors of response to chemotherapy
TL;DR: A group of miRNA predictors in response to chemosensitivity for CRC patients are identified, which could lead to a significant improvement in chemotherapy regimen selection strategy and personalized CRC management.
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