miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
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miR-145 suppresses cell invasion in hepatocellular carcinoma cells: miR-145 targets ADAM17
Xue-wei Yang,Long-juan Zhang,Xiao-Hui Huang,Lian-zhou Chen,Qiao Su,Wen-Tao Zeng,Wen Li,Qian Wang +7 more
TL;DR: The effect and mechanism of miR‐145 in the control of HCC cell invasion is explored to explore the effect and mode of action of this candidate tumor suppressor miRNA.
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The circRNA circP4HB promotes NSCLC aggressiveness and metastasis by sponging miR-133a-5p
TL;DR: Findings advocate targeting the circP4HB/miR-133a-5p/vimentin axis as a potential therapeutic option for NSCLC patients.
Journal ArticleDOI
Glutathione S-transferase P1 (GSTP1) suppresses cell apoptosis and its regulation by miR-133α in head and neck squamous cell carcinoma (HNSCC).
Muradil Mutallip,Nijiro Nohata,Toyoyuki Hanazawa,Naoko Kikkawa,Shigetoshi Horiguchi,Lisa Fujimura,Kazumori Kawakami,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Yoshitaka Okamoto,Naohiko Seki +11 more
TL;DR: The data indicate that GSTP1 may have an oncogenic function and may be regulated by miR-133α, a tumor suppressive miRNA in HNSCC, which could provide new insights into potential mechanisms of H NSCC carcinogenesis.
Journal ArticleDOI
SWAP70, actin-binding protein, function as an oncogene targeting tumor-suppressive miR-145 in prostate cancer†
Takeshi Chiyomaru,Shuichi Tatarano,Kazumori Kawakami,Hideki Enokida,Hirofumi Yoshino,Nijiro Nohata,Miki Fuse,Naohiko Seki,Masayuki Nakagawa +8 more
TL;DR: The aim of this study is to determine a novel oncogenic gene targeted by miR‐145 by focusing on actin‐binding proteins in CaP.
Journal ArticleDOI
miR-145 inhibits proliferation and invasion of esophageal squamous cell carcinoma in part by targeting c-Myc.
TL;DR: It is demonstrated that overexpression of miR-145 inhibits tumor growth in part by targeting c-Myc, and its dysregulation may be involved in the initiation and development of human ESCC.
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