miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
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LincRNA-ROR promotes metastasis and invasion of esophageal squamous cell carcinoma by regulating miR-145/FSCN1.
TL;DR: ROR acted as a competitive endogenous RNA (ceRNA) of miR-145 in ESCC and is likely an oncogene biomarker for ESCC early diagnosis and prognosis.
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miR-133b suppresses metastasis by targeting HOXA9 in human colorectal cancer.
Xiao Wang,Juyuan Bu,Xingwei Liu,Wenfeng Wang,Weihua Mai,Bao-Jun Lv,Jinlin Zou,Xiangqiong Mo,Xiaoling Li,Jingyu Wang,Bin Niu,Yunping Fan,Bingzong Hou +12 more
TL;DR: It is newly determined that miR-133b targeted the HOXA9/ZEB1 pathway to promote tumor metastasis in CRC cells.
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Reverse Correlation between MicroRNA-145 and FSCN1 Affecting Gastric Cancer Migration and Invasion
TL;DR: This study demonstrated that the first demonstration of the significant down-regulation of miR-145 expression in infiltrative gastric cancer compared to expanding Gastric cancer and directly targets FSCN1 and suppresses cell migration and invasion in gastriccancer.
Journal ArticleDOI
miR-133b inhibits glioma cell proliferation and invasion by targeting Sirt1.
TL;DR: Light is shed on the regulatory mechanism of miR-133b in glioma growth and metastasis via direct mediation of Sirt1 expression, and it is suggested that Sirt 1 may serve as a potential therapeutic target forglioma.
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A computationally constructed ceRNA interaction network based on a comparison of the SHEE and SHEEC cell lines.
Jiachun Sun,Junqiang Yan,Xiaozhi Yuan,Ruina Yang,Tanyou Dan,Xinshuai Wang,Guoqiang Kong,Shegan Gao +7 more
TL;DR: Functional pathway analyses demonstrated that the ceRNA network potentially modulated multiple signaling pathways, such as the MAPK, Ras, HIF-1, Rap1, and PI3K/Akt signaling pathways.
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