miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
Citations
More filters
Journal ArticleDOI
Exosome‐Mediated Transfer of miR‐133b from Multipotent Mesenchymal Stromal Cells to Neural Cells Contributes to Neurite Outgrowth
Hongqi Xin,Yi Li,Ben Buller,Mark Katakowski,Yi Zhang,Xinli Wang,Xia Shang,Zheng Gang Zhang,Michael Chopp,Michael Chopp +9 more
TL;DR: It is found that MSC treatment of rats subjected to middle cerebral artery occlusion significantly increased microRNA 133b (miR‐133b) level in the ipsilateral hemisphere and this study provides the first demonstration that M SCs communicate with brain parenchymal cells and may regulate neurite outgrowth by transfer of miR‐ 133b to neural cells via exosomes.
Journal ArticleDOI
The Somatic Genomic Landscape of Chromophobe Renal Cell Carcinoma
Caleb F. Davis,Christopher J. Ricketts,Min Wang,Lixing Yang,Andrew D. Cherniack,Hui Shen,Christian J. Buhay,Hyojin Kang,Sang Cheol Kim,Catherine C. Fahey,Kathryn E. Hacker,Gyan Bhanot,Dmitry A. Gordenin,Andy Chu,Preethi H. Gunaratne,Preethi H. Gunaratne,Michael Biehl,Sahil Seth,Benny Abraham Kaipparettu,Christopher A. Bristow,Lawrence A. Donehower,Eric Wallen,Angela B. Smith,Satish K. Tickoo,Pheroze Tamboli,Victor E. Reuter,Laura S. Schmidt,James J. Hsieh,James J. Hsieh,Toni K. Choueiri,A. Ari Hakimi,Chad J. Creighton,Margaret Morgan,David A. Wheeler,Richard A. Gibbs,Sabina Signoretti,A. Gordon Robertson,Elizabeth P. Henske,David J. Kwiatkowski,Christopher J. Ricketts,W. Marston Linehan,Michael P. Seiler,W. Kimryn Rathmell,Peter W. Laird,Brian Shuch,Donna M. Muzny,Hsu Chao,Mike Dahdouli,Liu Xi,Nipun Kakkar,Jeffrey G. Reid,Brittany Downs,Jennifer Drummond,Donna Morton,Harsha Doddapaneni,Lora Lewis,Adam C. English,Qingchang Meng,Christie Kovar,Qiaoyan Wang,Walker Hale,Alicia Hawes,Divya Kalra,Kimberly Walker,Bradley A. Murray,Carrie Sougnez,Gordon Saksena,Scott L. Carter,Steven E. Schumacher,Barbara Tabak,Travis I. Zack,Gad Getz,Rameen Beroukhim,Stacey Gabriel,Matthew Meyerson,Adrian Ally,Miruna Balasundaram,Inanc Birol,Denise Brooks,Yaron S.N. Butterfield,Eric Chuah,Amanda Clarke,Noreen Dhalla,Ranabir Guin,Robert A. Holt,Katayoon Kasaian,Darlene Lee,Haiyan I. Li,Emilia L. Lim,Yussanne Ma,Michael Mayo,Richard A. Moore,Andrew J. Mungall,Jacqueline E. Schein,Payal Sipahimalani,Angela Tam,Nina Thiessen,Tina Wong,Steven J.M. Jones,Marco A. Marra,J. Todd Auman,Donghui Tan,Shaowu Meng,Corbin D. Jones,Katherine A. Hoadley,Piotr A. Mieczkowski,Lisle E. Mose,Stuart R. Jefferys,Jeffrey Roach,Umadevi Veluvolu,Matthew D. Wilkerson,Scot Waring,Elizabeth Buda,Junyuan Wu,Tom Bodenheimer,Alan P. Hoyle,Janae V. Simons,Mathew G. Soloway,Saianand Balu,Joel S. Parker,D. Neil Hayes,Charles M. Perou,Daniel J. Weisenberger,Moiz S. Bootwalla,Timothy J. Triche,Phillip H. Lai,David Van Den Berg,Stephen B. Baylin,Fengju Chen,Cristian Coarfa,Michael S. Noble,Daniel DiCara,Hailei Zhang,Juok Cho,David I. Heiman,Nils Gehlenborg,Doug Voet,Pei Lin,Scott Frazer,Petar Stojanov,Yingchun Liu,Lihua Zou,Jaegil Kim,Michael S. Lawrence,Lynda Chin,Alexei Protopopov,Xingzhi Song,Jianhua Zhang,Angeliki Pantazi,Angela Hadjipanayis,Eunjung Lee,Lovelace J. Luquette,Semin Lee,Michael Parfenov,Netty Santoso,Jonathan G. Seidman,Andrew Wei Xu,Raju Kucherlapati,Peter J. Park,Junehawk Lee,Steven A. Roberts,Leszek J. Klimczak,David C. Fargo,Martin Lang,Yoon-La Choi,June Koo Lee,Woong-Yang Park,Wenyi Wang,Yu Fan,Jaeil Ahn,Rehan Akbani,John N. Weinstein,David Haussler,Singer Ma,Amie Radenbaugh,Jingchun Zhul,Tara M. Lichtenberg,Erik Zmuda,Aaron D. Black,Benjamin Hanf,Nilsa C. Ramirez,Lisa Wise,Jay Bowen,Kristen M. Leraas,Tracy M. Hall,Julie M. Gastier-Foster,William G. Kaelin,Leigh B. Thorne,Lori Boice,Mei Huang,Cathy D. Vocke,James Peterson,Robert Worrell,Maria J. Merino,Bogdan Czerniak,Kenneth Aldape,Christopher G. Wood,Paul T. Spellman,Michael B. Atkins,John C. Cheville,R. Houston Thompson,Mark A. Jensen,Todd Pihl,Yunhu Wan,Brenda Ayala,Julien Baboud,Sudhakar Velaga,Jessica Walton,Jia Liu,Sudha Chudamani,Ye Wu,Margi Sheth,Kenna R. Mills Shaw,John A. Demchok,Tanja M. Davidsen,Liming Yang,Zhining Wang,Roy Tarnuzzer,Jiashan Zhang,Greg Eley,Ina Felau,Jean C. Zenklusen,Carolyn M. Hutter,Mark S. Guyer,Bradley A. Ozenberger,Heidi J. Sofia +225 more
TL;DR: Genomic rearrangements lead to recurrent structural breakpoints within TERT promoter region, which correlates with highly elevated TERT expression and manifestation of kataegis, representing a mechanism of TERT upregulation in cancer distinct from previously observed amplifications and point mutations.
Journal ArticleDOI
A Role for miR-145 in Pulmonary Arterial Hypertension Evidence From Mouse Models and Patient Samples
Paola Caruso,Yvonne Dempsie,Hannah Stevens,Robert A. McDonald,Lu Long,Lu Long,Ruifang Lu,Kevin P. White,Kirsty M. Mair,John D. McClure,Mark Southwood,Mark Southwood,Paul D. Upton,Paul D. Upton,Mei Xin,Eva Van Rooij,Eric N. Olson,Nicholas W. Morrell,Nicholas W. Morrell,Margaret R. MacLean,Andrew H. Baker +20 more
TL;DR: In this article, the role of miR-145 in pulmonary arterial hypertension (PAH) was evaluated in mice treated with anti-miRs via measurement of systolic right ventricular pressure, right ventriches hypertrophy, and percentage of remodeled pulmonary arteries.
Journal ArticleDOI
The tumour-suppressive function of miR-1 and miR-133a targeting TAGLN2 in bladder cancer
Hirofumi Yoshino,Takeshi Chiyomaru,Hideki Enokida,Kazumori Kawakami,S. Tatarano,Kenryu Nishiyama,Nijiro Nohata,Naohiko Seki,Masayuki Nakagawa +8 more
TL;DR: The downregulation ofmiR-1 and miR-133a was a frequent event in BC, and these miRNAs were recognised as tumour suppressive and TAGLN2 may be a target of both mi RNAs and had a potential oncogenic function.
Journal ArticleDOI
Filopodia and adhesion in cancer cell motility
TL;DR: Data implicated that several different filopodia inducing genes may contribute in a collective manner to cancer progression and the high metastasis rates associated with basal-type breast carcinomas.
References
More filters
Journal ArticleDOI
Effect of surgical experience on results of esophagectomy for esophageal carcinoma.
TL;DR: The effect of surgical experience on results of esophagectomy has received little attention in the medical literature, but the association with substantial operative morbidity and mortality is significant.
Journal ArticleDOI
Protein alterations in ESCC and clinical implications: a review
TL;DR: This review summarizes all the dysregulated proteins investigated in the disease from 187 published papers and analyzes their contributions to tumor development and progression, in which cyclin D1, p53, E-cadherin and VEGF appeared to have the strongest evidence as independent predictors of patient outcome.
Journal ArticleDOI
Is combined chemotherapy and radiation therapy equally effective as surgical resection in localized esophageal carcinoma
Alexander Chan,Alfred Wong +1 more
TL;DR: Combined chemotherapy and radiation appeared to be as effective as esophagectomy in localized esophageal cancer.
Journal ArticleDOI
The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma
Sugimoto Takashi,Naohiko Seki,Satoya Shimizu,Naoko Kikkawa,Jun Tsukada,Hideaki Shimada,Keita Sasaki,Toyoyuki Hanazawa,Yoshitaka Okamoto,Akira Hata +9 more
TL;DR: GAL, coding for the neuropeptide Galanin, and GALR1, a galanin receptor, were identified as candidate genes of oncogenesis in SCC and their expression levels were confirmed by real‐time PCR.
Journal ArticleDOI
Expression of fascin, an actin-bundling protein, in astrocytomas of varying grades.
Aurelia Peraud,Soma Mondal,Cynthia Hawkins,Michelle Mastronardi,Kristy Bailey,James T. Rutka +5 more
TL;DR: This is the first study to demonstrate the expression of fascin in human astrocytic neoplasms, and the role that fascin plays in contributing to the invasive phenotype of anaplastic astROcytomas awaits further study and investigation.