miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
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CD47 expression regulated by the miR-133a tumor suppressor is a novel prognostic marker in esophageal squamous cell carcinoma.
Shigemasa Suzuki,Takehiko Yokobori,Naritaka Tanaka,Makoto Sakai,Akihiko Sano,Takanori Inose,Makoto Sohda,Masanobu Nakajima,Tatsuya Miyazaki,Hiroyuki Kato,Hiroyuki Kuwano +10 more
TL;DR: CD47 expression is a novel prognostic marker in ESCC that is directly inhibited by the miR-133a tumor suppressor and could provide new insight into the mechanism of cancer progression.
Journal ArticleDOI
Loss of miR-133a expression associated with poor survival of breast cancer and restoration of miR-133a expression inhibited breast cancer cell growth and invasion
Zheng Sheng Wu,Zheng Sheng Wu,Chao Qun Wang,Ru Xiang,Xue Liu,Shan Ye,Xue qing Yang,Gui Hong Zhang,Xiao Chun Xu,Xiao Chun Xu,Tao Zhu,Qiang Wu +11 more
TL;DR: miR-133a expression was lost in breast cancer tissues, loss of which was associated with lymph nodes metastasis, high clinical stages and shorter relapse-free survivals of patients with breast cancer.
Journal ArticleDOI
Tumor-suppressive microRNA-135a inhibits cancer cell proliferation by targeting the c-MYC oncogene in renal cell carcinoma.
Yasutoshi Yamada,Hideo Hidaka,Naohiko Seki,Hirofumi Yoshino,Takeshi Yamasaki,Toshihiko Itesako,Masayuki Nakagawa,Hideki Enokida +7 more
TL;DR: The data suggest that elucidation of tumor‐suppressive miR‐135a‐mediated molecular pathways could reveal potential therapeutic targets in RCC.
Journal ArticleDOI
HDAC Inhibitors Target HDAC5, Upregulate MicroRNA-125a-5p, and Induce Apoptosis in Breast Cancer Cells
Tsung Hua Hsieh,Chia Yi Hsu,Cheng Fang Tsai,Cheng-Yu Long,Chin Hu Wu,Deng-Chyang Wu,Jau Nan Lee,Wei Chun Chang,Eing-Mei Tsai +8 more
TL;DR: It is shown that HDACi reduce tumorigenesis and induce intrinsic apoptosis of human breast cancer cells through the microRNA miR-125a-5p in vivo and in vitro.
Journal ArticleDOI
MiR-203 suppresses tumor growth and invasion and down-regulates MiR-21 expression through repressing Ran in esophageal cancer.
Fang Zhang,Zhiping Yang,Minjun Cao,Yinsheng Xu,Jintao Li,Xuebin Chen,Zhi Gao,Jing Xin,Shaomei Zhou,Zhixiang Zhou,Yishu Yang,Wang Sheng,Yi Zeng +12 more
TL;DR: It is shown that overexpression of miR-203 in esophageal cancer cells dramatically increased cell apoptosis and inhibited cell proliferation, migration and invasion as well as tumor growth and down-regulated miR -21 expression, and small GTPase Ran was a target gene of mi R-203.
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