miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
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MicroRNA-133a, downregulated in osteosarcoma, suppresses proliferation and promotes apoptosis by targeting Bcl-xL and Mcl-1.
Fang Ji,Hao Zhang,Yang Wang,Ming Li,Weidong Xu,Yi-fan Kang,Zhiwei Wang,Zi-min Wang,Ping Cheng,Dake Tong,Cheng Li,Hao Tang +11 more
TL;DR: It is found that miR-133a was downregulated in osteosarcoma cell lines and primary human osteosARcoma tissues, and its decrease was significantly correlated with tumor progression and prognosis of the patients.
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Fascin: a key regulator of cytoskeletal dynamics.
Asier Jayo,Maddy Parsons +1 more
TL;DR: Fascin has received considerable attention recently as an emerging key prognostic marker of metastatic disease and to investigate fascin as a potential therapeutic target for a number of forms of cancer.
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MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF
Chao Zou,Qing Xu,Feng Mao,Dan Li,Chuan-Xiu Bian,Ling-Zhi Liu,Yue Jiang,Xiaona Chen,Yanting Qi,Xiaolong Zhang,Xuejing Wang,Qiang Sun,Hsiang-Fu Kung,Marie C. Lin,Marie C. Lin,Andreas Dress,Fiona C. Wardle,Bing-Hua Jiang,Lihui Lai +18 more
TL;DR: It is demonstrated that miR-145 plays important inhibitory role in breast cancer malignancy by targeting N-RAS and VEGF-A, which may be potential therapeutic and diagnostic targets.
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MicroRNA-145: a potent tumour suppressor that regulates multiple cellular pathways
TL;DR: This review aims to summarize the recent published literature on the role of microRNA‐145 in regulating tumourigenesis and progression, and explore its potential for cancer diagnosis, prognosis and treatment.
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Roles of the canonical myomiRs miR-1, -133 and -206 in cell development and disease.
TL;DR: The involvement of the related myomiRs at the crossroads between cell development/tissues regeneration/tissue inflammation responses, and cancer development is examined.
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