miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
Masayuki Kano,Naohiko Seki,Naoko Kikkawa,Lisa Fujimura,Isamu Hoshino,Yasunori Akutsu,Takeshi Chiyomaru,Hideki Enokida,Masayuki Nakagawa,Hisahiro Matsubara +9 more
TLDR
The identification of tumor‐suppressive miRNAs,miR‐145, miR‐133a and miR-133b, directly control oncogenic FSCN1 gene, and could provide new insights into potential mechanisms of ESCC carcinogenesis.Abstract:
MicroRNAs (miRNAs), noncoding RNAs 21–25 nucleotides in length, regulate gene expression primarily at the posttranscriptional level. Growing evidence suggests that miRNAs are aberrantly expressed in many human cancers, and that they play significant roles in carcinogenesis and cancer progression. A search for miRNAs with a tumor-suppressive function in esophageal squamous cell carcinoma (ESCC) was performed using the miRNA expression signatures obtained from ESCC clinical specimens. A subset of 15 miRNAs was significantly downregulated in ESCC. A comparison of miRNA signatures from ESCC and our previous report identified 4 miRNAs that are downregulated in common (miR-145, miR-30a-3p, miR-133a and miR-133b), suggesting that these miRNAs are candidate tumor suppressors. Gain-of-function analysis revealed that 3 transfectants (miR-145, miR-133a and miR-133b) inhibit cell proliferation and cell invasion in ESCC cells. These miRNAs (miR-145, miR-133a and miR-133b), which have conserved sequences in the 3′UTR of FSCN1 (actin-binding protein, Fascin homolog 1), inhibited FSCN1 expression. The signal from a luciferase reporter assay was significantly decreased at 2 miR-145 target sites and 1 miR-133a/b site, suggesting both miRNAs directly regulate FSCN1. An FSCN1 loss-of-function assay found significant cell growth and invasion inhibition, implying an FSCN1 is associated with ESCC carcinogenesis. The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis.read more
Citations
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References
More filters
Journal ArticleDOI
Identification of novel microRNA targets based on microRNA signatures in bladder cancer.
Takahiro Ichimi,Hideki Enokida,Yasushi Okuno,Ryo Kunimoto,Takeshi Chiyomaru,Ken Kawamoto,Kazuya Kawahara,Kazuki Toki,Kazumori Kawakami,Kenryu Nishiyama,Gozoh Tsujimoto,Masayuki Nakagawa,Naohiko Seki +12 more
TL;DR: The target search algorithm and gene‐expression profiling in BCs revealed that Keratin7 (KRT7) mRNA was a common target of the downregulated miRNAs, and the mRNA expression levels of KRT7 were significantly higher inBCs than in NBEs.
Journal ArticleDOI
Three-Field Lymph Node Dissection for Squamous Cell and Adenocarcinoma of the Esophagus
TL;DR: Esophagectomy with three-field lymph node dissection can be performed with a low mortality and reasonable morbidity, and an overall 5-year survival rate of 51% suggests a true survival benefit beyond that achieved solely on the basis of stage migration.
Journal ArticleDOI
MicroRNA Expression Profiles of Esophageal Cancer
Andrew Feber,Liqiang Xi,James D. Luketich,Arjun Pennathur,Rodney J. Landreneau,Maoxin Wu,Scott J. Swanson,Tony E. Godfrey,Virginia R. Litle +8 more
TL;DR: Examination of microRNA expression in Barrett esophagus and esophageal cancer to identify potential markers for disease progression revealed microRNAs that may be markers of tumor progression that can discriminate normal tissue from tumor.
Journal ArticleDOI
Roles of fascin in cell adhesion and motility.
TL;DR: Understanding of the multifaceted regulation of Fascin and the roles of fascin-containing structures in cell adhesion, motility and invasion in the life of vertebrate organisms is advanced.
Journal ArticleDOI
Distinctive MicroRNA Profiles Relating to Patient Survival in Esophageal Squamous Cell Carcinoma
Guo Yong,Zhaoli Chen,Liang Zhang,Fang Zhou,Su-sheng Shi,Xiaoli Feng,Baozhong Li,Xin Meng,Xi Ma,Mingyong Luo,Kang Shao,Ning Li,Bin Qiu,Keith Mitchelson,Jing Cheng,Jie He +15 more
TL;DR: The results indicate that microRNA expression profiles are important diagnostic and prognostic markers of esophageal cancer, which might be analyzed simply using economical approaches such as reverse transcription-PCR.