Journal ArticleDOI
Multiplexed electrical detection of cancer markers with nanowire sensor arrays.
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TLDR
Highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.Abstract:
We describe highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays. Protein markers were routinely detected at femtomolar concentrations with high selectivity, and simultaneous incorporation of control nanowires enabled discrimination against false positives. Nanowire arrays allowed highly selective and sensitive multiplexed detection of prostate specific antigen (PSA), PSA-a1-antichymotrypsin, carcinoembryonic antigen and mucin-1, including detection to at least 0.9 pg/ml in undiluted serum samples. In addition, nucleic acid receptors enabled real-time assays of the binding, activity and small-molecule inhibition of telomerase using unamplified extracts from as few as ten tumor cells. The capability for multiplexed real-time monitoring of protein markers and telomerase activity with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.read more
Citations
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Selected nanotechnologies and nanostructures for drug delivery, nanomedicine and cure.
TL;DR: This review article highlights the recent advances in nanostructures and nanotechnology for drug delivery, nanomedicine and cures.
Journal ArticleDOI
Preclinical predictors of clinical safety: opportunities for improvement.
TL;DR: Two goals of safe and efficient drug development must be to improve the human relevance of animal testing with new models and technologies, and to advance quickly to clinical testing armed with improved safety biomarker tools.
Journal ArticleDOI
Nanostructures Enabled by On-Wire Lithography (OWL).
TL;DR: Structures prepared by this method have provided valuable insight into the plasmonic properties of noble metal nanomaterials and have formed the basis for novel molecular electronic, encoding, and biological detection devices.
Journal ArticleDOI
Label-Free Multiplexed Electrical Detection of Cancer Markers on a Microchip Featuring an Integrated Fluidic Diode Nanopore Array
Lian Duan,Levent Yobas +1 more
TL;DR: An integrated array of glass nanopores on a silicon microchip fabricated in a batch process through low-resolution photolithography and standard semiconductor processing tools is introduced and ultrasensitive, label-free, multiplexed electrical detection of cancer-marker proteins in real time through charge-dependent ionic current rectification is demonstrated.
Journal ArticleDOI
Voltammetric detection of cancer biomarkers exemplified by interleukin-10 and osteopontin with silica nanowires
Niranjan S. Ramgir,Aleksandra Zajac,Praveen K. Sekhar,Latasha Lee,Tatyana A. Zhukov,Shekhar Bhansali +5 more
TL;DR: Real-time voltammetric detection of potential lung cancer biomarkers, namely, interleukin-10 (IL-10) and osteopontin (OPN), using localized silica nanowires as templates, through an electrochemical alkaline phosphatase (AP) based assay is reported.
References
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Journal ArticleDOI
Specific association of human telomerase activity with immortal cells and cancer
Nam Woo Kim,Mieczyslaw A. Piatyszek,Karen R. Prowse,Calvin B. Harley,Michael D. West,Peter L. C. Ho,Gina M. Coviello,Woodring E. Wright,Scott L. Weinrich,Jerry W. Shay +9 more
TL;DR: A highly sensitive assay for measuring telomerase activity was developed in this paper, which showed that telomerases appear to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.
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Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
TL;DR: The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
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In vivo cancer targeting and imaging with semiconductor quantum dots
TL;DR: Sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions are achieved and a whole-body macro-illumination system with wavelength-resolved spectral imaging is integrated for efficient background removal and precise delineation of weak spectral signatures.
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Cancer nanotechnology: opportunities and challenges.
TL;DR: Nanotechnology is a multidisciplinary field, which covers a vast and diverse array of devices derived from engineering, biology, physics and chemistry that can provide essential breakthroughs in the fight against cancer.
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The use of nanocrystals in biological detection
TL;DR: The emerging ability to control the patterns of matter on the nanometer length scale can be expected to lead to entirely new types of biological sensors capable of sensing at the single-molecule level in living cells, and capable of parallel integration for detection of multiple signals.