Journal ArticleDOI
Multiplexed electrical detection of cancer markers with nanowire sensor arrays.
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TLDR
Highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.Abstract:
We describe highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays. Protein markers were routinely detected at femtomolar concentrations with high selectivity, and simultaneous incorporation of control nanowires enabled discrimination against false positives. Nanowire arrays allowed highly selective and sensitive multiplexed detection of prostate specific antigen (PSA), PSA-a1-antichymotrypsin, carcinoembryonic antigen and mucin-1, including detection to at least 0.9 pg/ml in undiluted serum samples. In addition, nucleic acid receptors enabled real-time assays of the binding, activity and small-molecule inhibition of telomerase using unamplified extracts from as few as ten tumor cells. The capability for multiplexed real-time monitoring of protein markers and telomerase activity with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.read more
Citations
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Photocatalytic degradation of methylene blue using biosynthesized zinc oxide nanoparticles from bark extract of Kalopanax septemlobus
Jing Lu,Indra Batjikh,Joon Hurh,Yaxi Han,Hashmoonah Ali,Ramya Mathiyalagan,Chen Ling,Jong Chan Ahn,Deok-Chun Yang +8 more
TL;DR: In this article, the authors applied Kalopanax septemlobus-ZnO nanoparticles with green approach one-pot synthesis and found the presence of functional groups which as a capping agent and formation of ZnO NPs were confirmed in Fourier Transform Infrared Spectroscopy (FTIR) result.
Journal ArticleDOI
Design of surface modifications for nanoscale sensor applications.
Erik Reimhult,Fredrik Höök +1 more
TL;DR: How patterning of molecular films designed to control interactions of biomolecules with nanoscale biosensor surfaces creates new possibilities as well as new challenges is reviewed.
Journal ArticleDOI
Peptide-coated nanotube-based biosensor for the detection of disease-specific autoantibodies in human serum
Katerina A. Drouvalakis,Sarunya Bangsaruntip,Wolfgang Hueber,Leland G. Kozar,Paul J. Utz,Hongjie Dai +5 more
TL;DR: The sensitivity of the nanotube-based sensor was higher than that of the established ELISA and recently described microarray assay systems, and there was an 18.6% greater chance of a negative test being a true indicator of a person not having RA than by either ELISA or microarray.
Patent
DNA sequencing and amplification systems using nanoscale field effect sensor arrays
Rashid Bashir,Ashraf Alam,Demir Akin,Oguz H. Elibol,Bobby Reddy,Donald E. Bergstrom,Yi-Shao Liu +6 more
TL;DR: In this paper, field effect chemical sensor devices are used for chemical and/or biochemical sensing, and methods for single molecule detection are described. But they are not useful for amplification of target molecules by PCR.
Journal ArticleDOI
Strain in semiconductor core-shell nanowires
TL;DR: In this article, the authors used both continuum elasticity theory and an atomistic model to compute strain distributions in core-shell nanowires of zinc blende structure, and they found that the obtained strain distributions for infinite wires are excellent approximations to the strain distribution in finite wires, except in the regions close to the ends.
References
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Journal ArticleDOI
Specific association of human telomerase activity with immortal cells and cancer
Nam Woo Kim,Mieczyslaw A. Piatyszek,Karen R. Prowse,Calvin B. Harley,Michael D. West,Peter L. C. Ho,Gina M. Coviello,Woodring E. Wright,Scott L. Weinrich,Jerry W. Shay +9 more
TL;DR: A highly sensitive assay for measuring telomerase activity was developed in this paper, which showed that telomerases appear to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.
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Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
TL;DR: The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
Journal ArticleDOI
In vivo cancer targeting and imaging with semiconductor quantum dots
TL;DR: Sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions are achieved and a whole-body macro-illumination system with wavelength-resolved spectral imaging is integrated for efficient background removal and precise delineation of weak spectral signatures.
Journal ArticleDOI
Cancer nanotechnology: opportunities and challenges.
TL;DR: Nanotechnology is a multidisciplinary field, which covers a vast and diverse array of devices derived from engineering, biology, physics and chemistry that can provide essential breakthroughs in the fight against cancer.
Journal ArticleDOI
The use of nanocrystals in biological detection
TL;DR: The emerging ability to control the patterns of matter on the nanometer length scale can be expected to lead to entirely new types of biological sensors capable of sensing at the single-molecule level in living cells, and capable of parallel integration for detection of multiple signals.