Journal ArticleDOI
Multiplexed electrical detection of cancer markers with nanowire sensor arrays.
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TLDR
Highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.Abstract:
We describe highly sensitive, label-free, multiplexed electrical detection of cancer markers using silicon-nanowire field-effect devices in which distinct nanowires and surface receptors are incorporated into arrays. Protein markers were routinely detected at femtomolar concentrations with high selectivity, and simultaneous incorporation of control nanowires enabled discrimination against false positives. Nanowire arrays allowed highly selective and sensitive multiplexed detection of prostate specific antigen (PSA), PSA-a1-antichymotrypsin, carcinoembryonic antigen and mucin-1, including detection to at least 0.9 pg/ml in undiluted serum samples. In addition, nucleic acid receptors enabled real-time assays of the binding, activity and small-molecule inhibition of telomerase using unamplified extracts from as few as ten tumor cells. The capability for multiplexed real-time monitoring of protein markers and telomerase activity with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for diagnosis and treatment of cancer and other complex diseases.read more
Citations
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InAs quantum dot arrays decorating the facets of GaAs nanowires.
TL;DR: This new type of heterostructures opens a new avenue to the fabrication of highly efficient single-photon sources, novel quantum optics experiments, as well as the realization of intermediate-band nanowire solar cells for third-generation photovoltaics.
Journal ArticleDOI
Integrated Microfluidic Reactors.
TL;DR: This review summarizes recent progresses on the development of integrated microfluidics-based chemical reactors for parallel screening of in situ click chemistry libraries, multistep synthesis of radiolabeled imaging probes for positron emission tomography (PET), and sequential preparation of individually addressable conducting polymer nanowire (CPNW).
Journal ArticleDOI
Non plasmonic semiconductor quantum SERS probe as a pathway for in vitro cancer detection
TL;DR: The authors report on the development and testing of biocompatible semiconductor zinc oxide quantum probes on a platform for cell adhesion and analysis and demonstrate discrimination of cancerous and non-cancerous cells along with biomolecular sensing of DNA, RNA, proteins and lipids in vitro.
Journal ArticleDOI
A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood
TL;DR: An automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood that provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.
Journal ArticleDOI
Multiplexed plasmon sensor for rapid label-free analyte detection.
Christina Rosman,Janak Prasad,Andreas Neiser,Andreas Henkel,Jonathan Edgar,Carsten Sönnichsen +5 more
TL;DR: A novel multiplexed sensor with randomly deposited aptamer functionalized gold nanorods that demonstrates nanomolar sensitivity, sensor recycling, and the potential to upscale to hundreds or thousands of targets.
References
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Journal ArticleDOI
Specific association of human telomerase activity with immortal cells and cancer
Nam Woo Kim,Mieczyslaw A. Piatyszek,Karen R. Prowse,Calvin B. Harley,Michael D. West,Peter L. C. Ho,Gina M. Coviello,Woodring E. Wright,Scott L. Weinrich,Jerry W. Shay +9 more
TL;DR: A highly sensitive assay for measuring telomerase activity was developed in this paper, which showed that telomerases appear to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.
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Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
TL;DR: The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.
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In vivo cancer targeting and imaging with semiconductor quantum dots
TL;DR: Sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions are achieved and a whole-body macro-illumination system with wavelength-resolved spectral imaging is integrated for efficient background removal and precise delineation of weak spectral signatures.
Journal ArticleDOI
Cancer nanotechnology: opportunities and challenges.
TL;DR: Nanotechnology is a multidisciplinary field, which covers a vast and diverse array of devices derived from engineering, biology, physics and chemistry that can provide essential breakthroughs in the fight against cancer.
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The use of nanocrystals in biological detection
TL;DR: The emerging ability to control the patterns of matter on the nanometer length scale can be expected to lead to entirely new types of biological sensors capable of sensing at the single-molecule level in living cells, and capable of parallel integration for detection of multiple signals.