MXene-Graphene Field-Effect Transistor Sensing of Influenza Virus and SARS-CoV-2
Yanxiao Li,Zhekun Peng,Natalie J. Holl,Md. Rifat Hassan,John M. Pappas,Congjie Wei,Omid Hoseini Izadi,Yang Wang,Xiangyang Dong,Cheng Wang,Yue-Wern Huang,Dong-Hyun Kim,Chenglin Wu +12 more
- Vol. 6, Iss: 10, pp 6643-6653
TLDR
In this paper, an MXene-graphene field effect transistor (FET) sensor for both influenza virus and 2019-nCoV sensing was developed and characterized, which combines the high chemical sensitivity of MXene and the continuity of large-area high-quality graphene to form an ultra-sensitive virus-sensing transduction material (VSTM).Abstract:
An MXene-graphene field-effect transistor (FET) sensor for both influenza virus and 2019-nCoV sensing was developed and characterized. The developed sensor combines the high chemical sensitivity of MXene and the continuity of large-area high-quality graphene to form an ultra-sensitive virus-sensing transduction material (VSTM). Through polymer linking, we are able to utilize antibody-antigen binding to achieve electrochemical signal transduction when viruses are deposited onto the VSTM surface. The MXene-graphene VSTM was integrated into a microfluidic channel that can directly receive viruses in solution. The developed sensor was tested with various concentrations of antigens from two viruses: inactivated influenza A (H1N1) HA virus ranging from 125 to 250,000 copies/mL and a recombinant 2019-nCoV spike protein ranging from 1 fg/mL to 10 pg/mL. The average response time was about ∼50 ms, which is significantly faster than the existing real-time reverse transcription-polymerase chain reaction method (>3 h). The low limit of detection (125 copies/mL for the influenza virus and 1 fg/mL for the recombinant 2019-nCoV spike protein) has demonstrated the sensitivity of the MXene-graphene VSTM on the FET platform to virus sensing. Especially, the high signal-to-viral load ratio (∼10% change in source-drain current and gate voltage) also demonstrates the ultra-sensitivity of the developed MXene-graphene FET sensor. In addition, the specificity of the sensor was also demonstrated by depositing the inactivated influenza A (H1N1) HA virus and the recombinant 2019-nCoV spike protein onto microfluidic channels with opposite antibodies, producing signal differences that are about 10 times lower. Thus, we have successfully fabricated a relatively low-cost, ultrasensitive, fast-responding, and specific inactivated influenza A (H1N1) and 2019-nCoV sensor with the MXene-graphene VSTM.read more
Citations
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Journal ArticleDOI
Accuracy of novel antigen rapid diagnostics for SARS-CoV-2: A living systematic review and meta-analysis.
Lukas E. Brümmer,Stephan Katzenschlager,Mary Gaeddert,Christian Erdmann,Stephani Schmitz,Marc Bota,Maurizio Grilli,Jan Larmann,Markus A. Weigand,Nira R. Pollock,Aurélien Macé,Sergio Carmona,Stefano Ongarello,Jilian A. Sacks,Claudia M. Denkinger,Claudia M. Denkinger +15 more
TL;DR: A systematic review and meta-analysis of commercially available rapid diagnostic tests (Ag-RDTs) for SARS-CoV-2 up until 30 April 2021 was conducted in this paper.
Posted ContentDOI
The accuracy of novel antigen rapid diagnostics for SARS-CoV-2: a living systematic review and meta-analysis.
Lukas E. Brümmer,Stephan Katzenschlager,Mary Gaeddert,Christian Erdmann,Stephani Schmitz,Marc Bota,Maurizio Grilli,Jan Larmann,Markus A. Weigand,Nira R. Pollock,Sergio Carmona,Stefano Ongarello,Jilian A. Sacks,Claudia M. Denkinger +13 more
TL;DR: In this article, a systematic review and meta-analysis of commercially available rapid diagnostic tests (Ag-RDTs) is presented, where the clinical accuracy (sensitivity and specificity) of these tests are assessed.
The development of integrated circuits based on two-dimensional materials
Kaichen Zhu,Kaichen Zhu,Chao Wen,Areej Aljarb,Fei Xue,Xiangming Xu,Vincent Tung,Xixiang Zhang,Husam N. Alshareef,Mario Lanza +9 more
TL;DR: In this article, the development of integrated circuits based on 2D layered materials is examined and a roadmap for the future development is proposed to address the key challenges that need to be addressed to deliver highly scaled circuits.
Journal ArticleDOI
Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein
TL;DR: In this article, an electrochemical sensor based on a molecularly imprinted polymer synthetic receptor for the quantitative detection of SARS-CoV-2 spike protein subunit S1 (ncovS1), by harnessing the covalent interaction between 1,2-diols of the highly glycosylated protein and the boronic acid group of 3-aminophenylboronic acid (APBA), was presented.
Journal ArticleDOI
Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein
TL;DR: In this paper , an electrochemical sensor based on a molecularly imprinted polymer synthetic receptor for the quantitative detection of SARS-CoV-2 spike protein subunit S1 (ncovS1), by harnessing the covalent interaction between 1,2-diols of the highly glycosylated protein and the boronic acid group of 3-aminophenylboronic acid (APBA), was presented.
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