Novel method for rapid toxicity screening of magnetic nanoparticles
Alexander Erofeev,Petr V. Gorelkin,Anastasiia S Garanina,Anastasiia S Garanina,A. Alova,M. V. Efremova,M. V. Efremova,Nataliya S. Vorobyeva,Nataliya S. Vorobyeva,Christopher J Edwards,Yuri E. Korchev,Yuri E. Korchev,Alexander G. Majouga,Alexander G. Majouga,Alexander G. Majouga +14 more
Reads0
Chats0
TLDR
A label-free method for assessing nanoparticle toxicity using the rapid (less than 30 minutes) measurement of ROS with a novel nanoelectrode is developed.Abstract:
Iron oxide nanoparticles have attracted a great deal of research interest and have been widely used in bioscience and clinical research including as contrast agents for magnetic resonance imaging, hyperthermia and magnetic field assisted radionuclide therapy. It is therefore important to develop methods, which can provide high-throughput screening of biological responses that can predict toxicity. The use of nanoelectrodes for single cell analysis can play a vital role in this process by providing relatively fast, comprehensive, and cost-effective assessment of cellular responses. We have developed a new method for in vitro study of the toxicity of magnetic nanoparticles (NP) based on the measurement of intracellular reactive oxygen species (ROS) by a novel nanoelectrode. Previous studies have suggested that ROS generation is frequently observed with NP toxicity. We have developed a stable probe for measuring intracellular ROS using platinized carbon nanoelectrodes with a cavity on the tip integrated into a micromanipulator on an upright microscope. Our results show a significant difference for intracellular levels of ROS measured in HEK293 and LNCaP cancer cells before and after exposure to 10 nm size iron oxide NP. These results are markedly different from ROS measured after cell incubation with the same concentration of NP using standard methods where no differences have been detected. In summary we have developed a label-free method for assessing nanoparticle toxicity using the rapid (less than 30 minutes) measurement of ROS with a novel nanoelectrode.read more
Citations
More filters
Journal ArticleDOI
Real-Time Intracellular Measurements of ROS and RNS in Living Cells With Single Core-Shell Nanowire Electrodes
Xin-Wei Zhang,Quan-Fa Qiu,Hong Jiang,Fu-Li Zhang,Yan-Ling Liu,Christian Amatore,Christian Amatore,Wei-Hua Huang +7 more
TL;DR: A facile and robust solution to this problem based on a unique SiC-core-shell design to produce cylindrical NWEs with superior mechanical toughness provided by the SiC nano-core and an excellent electrochemical performance providing by the ultrathin carbon shell that can be used as such or platinized is presented.
Journal ArticleDOI
Synthesis, Properties and Applications of Magnetic Nanoparticles and Nanowires—A Brief Introduction
TL;DR: Magnetic nanoparticles and magnetic nano-species of complex topology (e.g., nanorods, nanowires, nanotubes, etc.) are overviewed briefly in the paper, mostly giving attention to the synthetic details and particle composition as mentioned in this paper.
Journal ArticleDOI
Analyzing the mechanisms of iron oxide nanoparticles interactions with cells: A road from failure to success in clinical applications.
Adam Frtús,Barbora Smolková,Mariia Uzhytchak,Mariia Lunova,Milan Jirsa,Šárka Kubinová,Alexandr Dejneka,Oleg Lunov +7 more
TL;DR: It is demonstrated that knowledge of cellular mechanisms of IONPs action helped to overcome certain translation problems in nanomedicine and provide a basement to increase the likelihood for clinical success of IONS, as well as propose outlook of how to overcome problems in the field.
Journal ArticleDOI
Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide.
Roman Akasov,N. V. Sholina,N. V. Sholina,D. A. Khochenkov,A. Alova,Petr V. Gorelkin,Alexander Erofeev,Alexander Erofeev,Alla N. Generalova,Evgeny V. Khaydukov,Evgeny V. Khaydukov,Evgeny V. Khaydukov +11 more
TL;DR: Non-toxic flavin mononucleotide (FMN), which is a water-soluble form of riboflavin (vitamin B2) as a promising agent for photodynamic therapy of melanoma is proposed and selective accumulation of FMN in melanoma cells in vivo and in vitro is demonstrated.
Journal ArticleDOI
In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs.
Alexander Vaneev,Alexander Vaneev,Petr V. Gorelkin,Anastasiia S Garanina,Helena Lopatukhina,Stepan Vodopyanov,Stepan Vodopyanov,A. Alova,A. Alova,Oxana Ryabaya,Roman Akasov,Roman Akasov,Yanjun Zhang,Yanjun Zhang,Pavel Novák,Pavel Novák,S. V. Salikhov,Maxim A. Abakumov,Yasufumi Takahashi,Christopher R. W. Edwards,Natalia L. Klyachko,Alexander G. Majouga,Alexander G. Majouga,Alexander G. Majouga,Yuri E. Korchev,Yuri E. Korchev,Alexander Erofeev,Alexander Erofeev +27 more
TL;DR: This work has shown that their new method can measure the ROS response to chemotherapy in tumor-bearing mice in real-time and provides an effective new approach for the measurement of intracellular ROS by platinized nanoelectrodes.
References
More filters
Journal ArticleDOI
Apoptosis: A Review of Programmed Cell Death
TL;DR: The goal of this review is to provide a general overview of current knowledge on the process of apoptosis including morphology, biochemistry, the role of apoptoses in health and disease, detection methods, as well as a discussion of potential alternative forms of apoptotic proteins.
Journal ArticleDOI
Toxic Potential of Materials at the Nanolevel
TL;DR: The establishment of principles and test procedures to ensure safe manufacture and use of nanomaterials in the marketplace is urgently required and achievable.
Journal ArticleDOI
Reactive oxygen species, cellular redox systems and apoptosis
Magdalena L. Circu,Tak Yee Aw +1 more
TL;DR: A full understanding of the redox control of apoptotic initiation and execution could underpin the development of therapeutic interventions targeted at oxidative stress-associated disorders.
Journal ArticleDOI
Role of reactive oxygen species (ROS) in apoptosis induction
TL;DR: The role of ROS in the regulation of apoptosis, especially in inflammatory cells, is focused on, with particular attention to mitochondria.
Journal ArticleDOI
Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species.
C. Carlson,Saber M. Hussain,Amanda M. Schrand,Laura K. Braydich-Stolle,K. L. Hess,R. L. Jones,John J. Schlager +6 more
TL;DR: A size-dependent toxicity was produced by silver nanoparticles, and one predominant mechanism of toxicity was found to be largely mediated through oxidative stress.
Related Papers (5)
Electrochemical Nanoprobes for Single-Cell Analysis
Paolo Actis,Sergiy Tokar,Jan Clausmeyer,Babak Babakinejad,Sofya Mikhaleva,Renaud Cornut,Yasufumi Takahashi,Ainara López Córdoba,Pavel Novák,Andrew Shevchuck,Jennifer A. Dougan,Sergei G. Kazarian,Petr V. Gorelkin,Alexander Erofeev,Igor V. Yaminsky,Patrick R. Unwin,Wolfgang Schuhmann,David Klenerman,Dmitri A. Rusakov,Elena V. Sviderskaya,Yuri E. Korchev +20 more