Open AccessJournal Article
PCR-Restriction Fragment Length Polymorphism analysis of fljB gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians.
TLDR
The results of the present study showed that fljB gene is highly conserved among avians in different geographical regions, suggesting not only the importance of flJB gene in survival of organism in different environmental conditions but also the relation between proteins encoded by flj B gene and serotyping scheme.Abstract:
Background and Objectives: Economic constraint of diseases arising from Salmonella Typhimurium causes the study of this zoonotic organism more important. Most studies on identification and characterization of S. Typhimurium are conducted at DNA level. Flagellin genes (fliC and fljB genes encoding phase-1 and phase-2 flagella, respectively) are useful as a model system for studying genetic differentiation. The objectives of the present study were to identify the polymorphism of fljB among avians in different regions by the PCR-RFLP method. Materials and Methods: Fifty-two S. Typhimurium isolates out of 1,870 intestine samples were identified using culture and serotyping as well as multiplex-PCR (broiler (n = 13), layer (n = 12), duck (n = 5), goose (n = 5), sparrow (n = 8), canary (n = 3), pigeon (n = 5) and casco parrot (n = 1) ). Amplification of fljB gene was performed and amplified products subjected to restriction digestion with Hha I enzyme. Results: Two RFLP patterns generated DNA fragments between approximately 50 to 800 bps. Pattern A was observed in 33 (63.46%) and pattern B in 19 (36.54%) of isolates. Salmonella Typhimurium recovered from 13 broilers (ten with pattern A and 3 with pattern B) and 8 sparrow (three with pattern A and 5 with pattern B) showed both A and B patterns. Twelve layers, 5 pigeons and 3 canaries showed pattern A and 5 ducks, 5 geese and one casco parrot showed pattern B. None of these patterns was allotted for a special region. Conclusion: The results of the present study showed that fljB gene is highly conserved among avians in different geographical regions, suggesting not only the importance of fljB gene in survival of organism in different environmental conditions but also the relation between proteins encoded by fljB gene and serotyping scheme.read more
Citations
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Antibiotic-Resistance Patterns of Gram-Negative Bacterial Isolates From Breeder Canaries (Serinus canaria domestica) With Clinical Disease
TL;DR: The results confirm the potential presence of multidrug-resistant bacteria in canary facilities, suggesting that measures to educate the public about this risk are necessary.
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Sequence-Level and Dual-Phase Identification of Salmonella Flagellum Antigens by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Keding Cheng,Keding Cheng,Angela Sloan,Julie Meakin,Stuart McCorrister,Morganne Jerome,Garrett Westmacott,Mike Drebot,Celine Nadon,J. David Knox,Gehua Wang +10 more
TL;DR: The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.
Journal ArticleDOI
PCR-RFLP analysis of fliC, fimH and 16S rRNA genes in Salmonella Typhimurium isolates of varied origin
T.G. Sumithra,Vinod Kumar Chaturvedi,Praveen K. Gupta,A. K. Rai,Sunita Chougule,Bincy Joseph,Susan Cherian,Chabi Lal Patel +7 more
TL;DR: The results demonstrate that PCR-RFLP based on these genes showed good typeability but low discriminatory power and suggests the importance of these genes as immunization and diagnostic factors in Salmonella Typhimurium.
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Identification of Avian Salmonella Isolates by PCR-RFLP Analysis of a fliC Gene Fragment
TL;DR: It is demonstrated that using HhaI restriction endonuclease could differentiate Salmonella enteritidis andSalmonella colindale but there is similarity between pattern of SalmoneLLA typhimurium and Salmoneella infantis.
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High-Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis
TL;DR: This high-throughput multiplex PCR assay allowed simple and accurate typing of the most prevalent clinical serovars of Salmonella enterica at a level comparable to that of conventional serotyping, but at a fraction of both the cost and time required per test.
Journal ArticleDOI
Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles.
Yang Hong,Tongrui Liu,Tongrui Liu,Margie D. Lee,Charles L. Hofacre,Marie Maier,Marie Maier,David G. White,Sherry Ayers,Lihua Wang,Roy D. Berghaus,John J. Maurer +11 more
TL;DR: Multiplex PCR assays for detecting specific O and H antigen gene alleles can be a rapid and cost-effective alternative approach to classical serotyping for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.
Journal ArticleDOI
Towards the development of a DNA-sequence based approach to serotyping of Salmonella enterica
TL;DR: FliC sequences were obtained for more than 100 strains comprising 29 different H1 alleles and unique pyrosequencing profiles corresponding to the H1 component of the serotype were generated reproducibly for 23 alleles represented in the evaluation panel.
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Detection and Identification of Salmonella Typhimurium in Bovine Diarrhoeic Fecal Samples by Immunomagnetic Separation and Multiplex PCR Assay
TL;DR: The results of this study showed that detection of Salmonella at genus level with universal ST139‐141 primers and identification ofSalmonella Typhimurium by using specific primers of O4, H2:1, 2 and H1 antigens can potentially permit to more readily evaluate fecal and other types of samples for the presence of these organisms.