Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells.
Arianna Dellavalle,Maurilio Sampaolesi,Rossana Tonlorenzi,Enrico Tagliafico,Benedetto Sacchetti,Laura Perani,Anna Innocenzi,Beatriz G. Gálvez,Graziella Messina,Roberta Morosetti,Sheng Li,Marzia Belicchi,Giuseppe M. Peretti,Jeffrey S. Chamberlain,Woodring E. Wright,Yvan Torrente,Stefano Ferrari,Paolo Bianco,Giulio Cossu +18 more
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TLDR
Data show that myogenic precursors, distinct from satellite cells, are associated with microvascular walls in the human skeletal muscle, may represent a correlate of embryonic 'mesoangioblasts' present after birth and may be a promising candidate for future cell-therapy protocols in patients.Abstract:
Cells derived from blood vessels of human skeletal muscle can regenerate skeletal muscle, similarly to embryonic mesoangioblasts. However, adult cells do not express endothelial markers, but instead express markers of pericytes, such as NG2 proteoglycan and alkaline phosphatase (ALP), and can be prospectively isolated from freshly dissociated ALP(+) cells. Unlike canonical myogenic precursors (satellite cells), pericyte-derived cells express myogenic markers only in differentiated myotubes, which they form spontaneously with high efficiency. When transplanted into severe combined immune deficient-X-linked, mouse muscular dystrophy (scid-mdx) mice, pericyte-derived cells colonize host muscle and generate numerous fibres expressing human dystrophin. Similar cells isolated from Duchenne patients, and engineered to express human mini-dystrophin, also give rise to many dystrophin-positive fibres in vivo. These data show that myogenic precursors, distinct from satellite cells, are associated with microvascular walls in the human skeletal muscle, may represent a correlate of embryonic 'mesoangioblasts' present after birth and may be a promising candidate for future cell-therapy protocols in patients.read more
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A perivascular origin for mesenchymal stem cells in multiple human organs
Mihaela Crisan,Solomon Yap,Louis Casteilla,Louis Casteilla,Chien Wen Chen,Mirko Corselli,Tea Soon Park,Gabriella Andriolo,Bin Sun,Bo Zheng,Li Zhang,Cyrille Norotte,Pang-ning Teng,Jeremy Traas,Rebecca C. Schugar,Bridget M. Deasy,Stephen F. Badylak,Hans-Jörg Bühring,Jean-Paul Giacobino,Lorenza Lazzari,Johnny Huard,Bruno Péault +21 more
TL;DR: Blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
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Pericytes: developmental, physiological, and pathological perspectives, problems, and promises.
TL;DR: The history of investigations into pericytes, the mural cells of blood microvessels, are reviewed, emerging concepts are indicated, and problems and promise are pointed out.
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Satellite Cells and the Muscle Stem Cell Niche
TL;DR: For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved the understanding of skeletal muscle biology, with focuses on functions of satellite cells and their niche during the process ofletal muscle regeneration.
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Mesenchymal Stem Cells: Revisiting History, Concepts, and Assays
TL;DR: The experimental origin of the concept of the population(s) referred to as mesenchymal stem cells and the experimental framework required to assess their stemness and function are revisited.
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The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine
Paolo Bianco,Xu Cao,Paul S. Frenette,Jeremy J. Mao,Pamela Gehron Robey,Paul J. Simmons,Cun-Yu Wang +6 more
TL;DR: Significant ambiguities still plague the field regarding the nature, identity, function, mode of isolation and experimental handling of MSCs, which have a major impact on their envisioned therapeutic use.
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