Phosphorylation of 1-Aminocyclopropane-1-Carboxylic Acid Synthase by MPK6, a Stress-Responsive Mitogen-Activated Protein Kinase, Induces Ethylene Biosynthesis in Arabidopsis
Yidong Liu,Shuqun Zhang +1 more
Reads0
Chats0
TLDR
The identification of the first plant MAPK substrate in this report reveals one mechanism by which MPK6/SIPK regulates plant stress responses and uncovers a signaling pathway that modulates the biosynthesis of ethylene, an important plant hormone, in plants under stress.Abstract:
Mitogen-activated protein kinases (MAPKs) are implicated in regulating plant growth, development, and response to the environment. However, the underlying mechanisms are unknown because of the lack of information about their substrates. Using a conditional gain-of-function transgenic system, we demonstrated that the activation of SIPK, a tobacco (Nicotiana tabacum) stress-responsive MAPK, induces the biosynthesis of ethylene. Here, we report that MPK6, the Arabidopsis thaliana ortholog of tobacco SIPK, is required for ethylene induction in this transgenic system. Furthermore, we found that selected isoforms of 1-aminocyclopropane-1-carboxylic acid synthase (ACS), the rate-limiting enzyme of ethylene biosynthesis, are substrates of MPK6. Phosphorylation of ACS2 and ACS6 by MPK6 leads to the accumulation of ACS protein and, thus, elevated levels of cellular ACS activity and ethylene production. Expression of ACS6DDD, a gain-of-function ACS6 mutant that mimics the phosphorylated form of ACS6, confers constitutive ethylene production and ethylene-induced phenotypes. Increasing numbers of stress stimuli have been shown to activate Arabidopsis MPK6 or its orthologs in other plant species. The identification of the first plant MAPK substrate in this report reveals one mechanism by which MPK6/SIPK regulates plant stress responses. Equally important, this study uncovers a signaling pathway that modulates the biosynthesis of ethylene, an important plant hormone, in plants under stress.read more
Citations
More filters
Journal ArticleDOI
Plant immunity: towards an integrated view of plant―pathogen interactions
Peter N. Dodds,John P. Rathjen +1 more
TL;DR: The recent convergence of molecular studies of plant immunity and pathogen infection strategies is revealing an integrated picture of the plant–pathogen interaction from the perspective of both organisms, suggesting novel biotechnological approaches to crop protection.
Journal ArticleDOI
Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks
Miki Fujita,Yasunari Fujita,Yoshiteru Noutoshi,Fuminori Takahashi,Yoshihiro Narusaka,Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki +6 more
TL;DR: Emerging evidence suggests that hormone signaling pathways regulated by abscisic acid, salicylic acid, jasmonic acid and ethylene, as well as ROS signaling pathways, play key roles in the crosstalk between biotic and abiotic stress signaling.
Journal ArticleDOI
Mitogen-Activated Protein Kinase Signaling in Plants
TL;DR: Genetic analyses have uncovered several predominant MAPK components shared by several of these processes including the Arabidopsis thaliana MAPKs MPK3, 4, and 6 and MAP2Ks MKK1, 2, 3, and 5, and future work needs to focus on identifying substrates ofMAPKs, and on understanding how specificity is achieved among MAPK signaling pathways.
Journal ArticleDOI
MAPK cascades in plant disease resistance signaling.
Xiangzong Meng,Shuqun Zhang +1 more
TL;DR: Recent advances in the identification of upstream receptors/sensors and downstream MAPK substrates revealed the molecular mechanisms underlying MAPK functions in plant disease resistance and emerged as battlegrounds of plant-pathogen interactions.
Journal ArticleDOI
Promotion of Plant Growth by Bacterial ACC Deaminase
Bernard R. Glick,Biljana Todorovic,Jennifer Czarny,Zhenyu Cheng,Jin Duan,Brendan J. McConkey +5 more
TL;DR: A review of the published work on the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, with an emphasis on its biochemistry, protein structure, genes, and regulation.
References
More filters
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Salt and drought stress signal transduction in plants
TL;DR: Salt and drought stress signal transduction consists of ionic and osmotic homeostasis signaling pathways, detoxification (i.e., damage control and repair) response pathways, and pathways for growth regulation.
Journal ArticleDOI
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Jose M. Alonso,Anna Stepanova,Thomas J. Leisse,Christopher J. Kim,Huaming Chen,Paul Shinn,Denise K. Stevenson,Justin Zimmerman,Pascual Barajas,Rosa Cheuk,Carmelita Gadrinab,Collen Heller,Albert Jeske,Eric Koesema,Cristina C. Meyers,Holly Parker,Lance Prednis,Yasser Ansari,Nathan Choy,Hashim Deen,Michael Geralt,Nisha Hazari,Emily Hom,Meagan Karnes,Celene Mulholland,Ral Ndubaku,Ian Thomas Schmidt,Plinio Guzmán,Laura Aguilar-Henonin,Markus Schmid,Detlef Weigel,David E. Carter,Trudy Marchand,Eddy Risseeuw,Debra Brogden,Albana Zeko,William L. Crosby,Charles C. Berry,Joseph R. Ecker +38 more
TL;DR: Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels, and insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
Journal ArticleDOI
Mammalian MAP kinase signalling cascades
Lufen Chang,Michael Karin +1 more
TL;DR: Recent studies have begun to shed light on the physiological functions of MAPK cascades in the control of gene expression, cell proliferation and programmed cell death.
Journal ArticleDOI
Signal transduction by the JNK group of MAP kinases.
TL;DR: This review will focus on the JNK group of MAP kinases, which are characterized by the sequence TEY and the two stress-activatedMAP kinases: p38 with the sequence TGY, and the c-Jun NH2-terminal kinases (JNK) with the sequences TPY.
Related Papers (5)
Mitogen-activated protein kinase cascades in plants: a new nomenclature
Kazuya Ichimura,Kazuo Shinozaki,Guillaume Tena,Jen Sheen,Yves Henry,Anthony Champion,Martin Kreis,Shuqun Zhang,Heribert Hirt,Cathal Wilson,Erwin Heberle-Bors,Brian E. Ellis,Peter Morris,Roger W. Innes,Joseph R. Ecker,Dierk Scheel,Daniel F. Klessig,Yasunori Machida,John Mundy,Yuko Ohashi,John C. Walker +20 more