Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules.
Sarah A. Wilson,Susan C. Roberts +1 more
TLDR
Recent advancements in plant cell culture processing technology are discussed, focusing on progress towards overcoming the problems associated with commercialization of these production systems and highlighting recent commercial successes.Abstract:
Plant cell culture systems were initially explored for use in commercial synthesis of several high-value secondary metabolites, allowing for sustainable production that was not limited by the low yields associated with natural harvest or the high cost associated with complex chemical synthesis. Although there have been some commercial successes, most notably paclitaxel production from Taxus sp., process limitations exist with regards to low product yields and inherent production variability. A variety of strategies are being developed to overcome these limitations including elicitation, in situ product removal and metabolic engineering with single genes and transcription factors. Recently, the plant cell culture production platform has been extended to pharmaceutically active heterologous proteins. Plant systems are beneficial because they are able to produce complex proteins that are properly glycosylated, folded and assembled without the risk of contamination by toxins that are associated with mammalian or microbial production systems. Additionally, plant cell culture isolates transgenic material from the environment, allows for more controllable conditions over field-grown crops and promotes secretion of proteins to the medium, reducing downstream purification costs. Despite these benefits, the increase in cost of heterologous protein synthesis in plant cell culture as opposed to field-grown crops is significant and therefore processes must be optimized with regard to maximizing secretion and enhancing protein stability in the cell culture media. This review discusses recent advancements in plant cell culture processing technology, focusing on progress towards overcoming the problems associated with commercialization of these production systems and highlighting recent commercial successes.read more
Citations
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Discovery and resupply of pharmacologically active plant-derived natural products: A review.
Atanas G. Atanasov,Birgit Waltenberger,Eva-Maria Pferschy-Wenzig,Thomas Linder,Christoph Wawrosch,Pavel Uhrin,Veronika Temml,Limei Wang,Stefan Schwaiger,Elke H. Heiss,Judith M. Rollinger,Judith M. Rollinger,Daniela Schuster,Johannes M. Breuss,Valery N. Bochkov,Marko D. Mihovilovic,Brigitte Kopp,Rudolf Bauer,Verena M. Dirsch,Hermann Stuppner +19 more
TL;DR: While the intrinsic complexity of natural product-based drug discovery necessitates highly integrated interdisciplinary approaches, the reviewed scientific developments, recent technological advances, and research trends clearly indicate that natural products will be among the most important sources of new drugs in the future.
Journal ArticleDOI
Recent advances and uses of grape flavonoids as nutraceuticals.
TL;DR: The recent scientific advances supporting the beneficial health qualities of grape and grape-derived products, mechanisms of their biological activity, bioavailability, and their uses as nutraceuticals are discussed.
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In vitro plant tissue culture: means for production of biological active compounds
TL;DR: This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.
Journal ArticleDOI
Plant cell culture strategies for the production of natural products
Marisol Ochoa-Villarreal,Susan Howat,Sunmi Hong,Mi Ok Jang,Young-Woo Jin,Eun-Kyong Lee,Gary J. Loake +6 more
TL;DR: There remain significant obstacles to the commercial synthesis of high-value chemicals from these sources but the relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.
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Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro
TL;DR: Several synthetic biology methods to enhance and diversify the production of plant terpenoids are highlighted, with a foresight towards triterpenoid engineering, the least engineered class of bioactive ter penoids.
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An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
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Journal ArticleDOI
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
TL;DR: The effects of 5′ deletions in a plant viral promoter in tobacco callus as well as in regenerated plants, includ ing different plant tissues, are analysed to allow a more direct assessment of deletion effects.
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