Journal ArticleDOI
Regulated ATP release from astrocytes through lysosome exocytosis
Zhijun Zhang,Gang Chen,Gang Chen,Wei Zhou,Wei Zhou,Aihong Song,Tao Xu,Qingming Luo,Wei Wang,Xiaosong Gu,Shumin Duan +10 more
TLDR
It is found that lysosomes contain abundant ATP, which could be released in a stimulus-dependent manner and implicating physiological and pathological functions of regulated lysOSome exocytosis in these cells.Abstract:
Release of ATP from astrocytes is required for Ca2+ wave propagation among astrocytes and for feedback modulation of synaptic functions. However, the mechanism of ATP release and the source of ATP in astrocytes are still not known. Here we show that incubation of astrocytes with FM dyes leads to selective labelling of lysosomes. Time-lapse confocal imaging of FM dye-labelled fluorescent puncta, together with extracellular quenching and total-internal-reflection fluorescence microscopy (TIRFM), demonstrated directly that extracellular ATP or glutamate induced partial exocytosis of lysosomes, whereas an ischaemic insult with potassium cyanide induced both partial and full exocytosis of these organelles. We found that lysosomes contain abundant ATP, which could be released in a stimulus-dependent manner. Selective lysis of lysosomes abolished both ATP release and Ca2+ wave propagation among astrocytes, implicating physiological and pathological functions of regulated lysosome exocytosis in these cells.read more
Citations
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References
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Plasma Membrane Repair Is Mediated by Ca2+-Regulated Exocytosis of Lysosomes
TL;DR: It is shown that Ca(2+)-regulated exocytosis of lysosomes is required for the repair of plasma membrane disruptions and mediates the resealing of primary skin fibroblasts wounded during the contraction of collagen matrices.
Journal ArticleDOI
ATP Released from Astrocytes Mediates Glial Calcium Waves
Peter Guthrie,Joshua Knappenberger,Menahem Segal,Michael V. L. Bennett,Andrew Charles,S. B. Kater +5 more
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Journal ArticleDOI
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