Regulation of protein tyrosine phosphatases by reversible oxidation
Reads0
Chats0
TLDR
The role of PTP oxidation for physiological signalling processes as well as in different pathologies is described on the basis of well-investigated examples and criteria to establish the causal involvement of P TP oxidation in a given process are proposed.Abstract:
Oxidation of the catalytic cysteine of protein-tyrosine phosphatases (PTP), which leads to their reversible inactivation, has emerged as an important regulatory mechanism linking cellular tyrosine phosphorylation and signalling by reactive-oxygen or -nitrogen species (ROS, RNS). This review focuses on recent findings about the involved pathways, enzymes and biochemical mechanisms. Both the general cellular redox state and extracellular ligand-stimulated ROS production can cause PTP oxidation. Members of the PTP family differ in their intrinsic susceptibility to oxidation, and different types of oxidative modification of the PTP catalytic cysteine can occur. The role of PTP oxidation for physiological signalling processes as well as in different pathologies is described on the basis of well-investigated examples. Criteria to establish the causal involvement of PTP oxidation in a given process are proposed. A better understanding of mechanisms leading to selective PTP oxidation in a cellular context, and finding ways to pharmacologically modulate these pathways are important topics for future research.read more
Citations
More filters
Journal ArticleDOI
Modern Principles of Classification and Development of Nutrient Media for Culturing of Human and Animal Cells
TL;DR: Analysis of the main principles of classifi cation and development of nutrient media used for culturing of human and animal cells in biology and medicine and practical results obtained by the authors in the development of diverse purpose nutrient media are presented.
Journal ArticleDOI
Activated Thiol Sepharose-based proteomic approach to quantify reversible protein oxidation
TL;DR: An Activated Thiol Sepharose‐based proteomic approach to quantify reversible protein oxidation and can detect reversible oxidation of reactive cysteines and can be employed to analyze multiple physiologic and pathologic conditions.
Journal ArticleDOI
Context-dependent regulation of receptor tyrosine kinases: Insights from systems biology approaches.
TL;DR: Three concrete examples are described that illustrate how systems approaches can reveal key mechanistic and therapeutic insights in RTK networks, including ErbB3, VEGFR2, and AXL.
Journal ArticleDOI
Chronic exposure of the RAW246.7 macrophage cell line to H2O2 leads to increased catalase expression.
Robert N. Goddu,Catherine F. Henderson,Amanda K. Young,Brooke E. Muradian,Leticia Calderon,Logan H. Bleeg,Jon M. Fukuto,Joseph Lin +7 more
TL;DR: The hypothesis that macrophages are able to protect themselves and neighboring cells during states of chronic inflammation from the oxidizing environment they create is supported.
References
More filters
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal ArticleDOI
Protein Tyrosine Phosphatases in the Human Genome
Andres Alonso,Joanna Sasin,Nunzio Bottini,Ilan Friedberg,Iddo Friedberg,Andrei L. Osterman,Adam Godzik,Tony Hunter,Jack E. Dixon,Tomas Mustelin +9 more
TL;DR: The set of 107 genes in the human genome that encode members of the four protein tyrosine phosphatase (PTP) families are presented and the role of these enzymes in human disease will be discussed.
Journal ArticleDOI
Reactive Oxygen Species Promote TNFα-Induced Death and Sustained JNK Activation by Inhibiting MAP Kinase Phosphatases
TL;DR: It is shown that TNFalpha-induced ROS, whose accumulation is suppressed by mitochondrial superoxide dismutase, cause oxidation and inhibition of JNK-inactivating phosphatases by converting their catalytic cysteine to sulfenic acid, which results in sustained JNK activation, which is required for cytochrome c release and caspase 3 cleavage.
Journal ArticleDOI
Protein tyrosine phosphatases: from genes, to function, to disease
TL;DR: Recent breakthroughs in understanding of the role of the PTPs in the regulation of signal transduction and the aetiology of human disease are described.
Journal ArticleDOI
Hydrogen Peroxide Sensing and Signaling
TL;DR: The molecular mechanisms by which hydrogen peroxide is sensed and the increasing evidence that antioxidant enzymes play multiple, key roles as sensors and regulators of signal transduction in response to hydrogen peroxy are discussed.