Response of the Hyperthermophilic Archaeon Sulfolobus solfataricus to UV Damage
Sabrina Fröls,Paul M. K. Gordon,Mayi Arcellana Panlilio,Iain G. Duggin,Stephen D. Bell,Christoph Wilhelm Sensen,Christa Schleper +6 more
TLDR
In this paper, high-density DNA microarrays were used to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage.Abstract:
In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements. While no significant up-regulation of genes potentially involved in direct DNA damage reversal was observed, a specific transcriptional UV response involving 55 genes could be dissected. Although flow cytometry showed only modest perturbation of the cell cycle, strong modulation of the transcript levels of the Cdc6 replication initiator genes was observed. Up-regulation of an operon encoding Mre11 and Rad50 homologs pointed to induction of recombinational repair. Consistent with this, DNA double-strand breaks were observed between 2 and 8 h after UV treatment, possibly resulting from replication fork collapse at damaged DNA sites. The strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material. In support of this, a statistical microscopic analysis demonstrated that large cell aggregates formed upon UV exposure. Together, this provided supporting evidence to a link between recombinational repair and conjugation events.read more
Citations
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The archaeal cell envelope
TL;DR: The wide range of cell wall polymers, O- and N-glycosylated extracellular proteins and other cell surface structures that archaea use to interact with their environment are described.
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A unique cell division machinery in the Archaea
TL;DR: Two of the Cdv proteins display homology to components of the eukaryotic ESCRT-III sorting complex involved in budding of luminal vesicles and HIV-1 virion release, suggesting mechanistic similarities and a common evolutionary origin.
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UV‐inducible cellular aggregation of the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by pili formation
Sabrina Fröls,Malgorzata Ajon,Michaela Wagner,Daniela Teichmann,Behnam Zolghadr,Mihaela Folea,Egbert J. Boekema,Arnold J. M. Driessen,Christa Schleper,Christa Schleper,Sonja-Verena Albers +10 more
TL;DR: It is hypothesized that pili formation and subsequent cellular aggregation enhance DNA transfer among Sulfolobus cells to provide increased repair of damaged DNA via homologous recombination.
Journal ArticleDOI
Responses of hyperthermophilic crenarchaea to UV irradiation
TL;DR: A clear response to DNA damage was observed, with down- regulation of the DNA replication machinery, changes in transcriptional regulatory proteins, and up-regulation of the biosynthetic enzymes for beta-carotene, which has UV protective properties, and proteins that detoxify reactive oxygen species.
Journal ArticleDOI
UV-inducible DNA Exchange in Hyperthermophilic Archaea Mediated by Type IV Pili
Malgorzata Ajon,Sabrina Fröls,Marleen van Wolferen,Kilian Stoecker,Daniela Teichmann,Arnold J. M. Driessen,Dennis W. Grogan,Sonja-Verena Albers,Christa Schleper +8 more
TL;DR: It is demonstrated by fluorescence in situ hybridization that cellular aggregates are formed based on a species‐specific recognition process and that UV‐induced cellular aggregation mediates chromosomal marker exchange with high frequency and may contribute substantially to the frequent chromosomal DNA exchange and horizontal gene transfer in these archaea in their natural habitat.
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