Journal ArticleDOI
Single cell detection with micromechanical oscillators
Bojan Ilic,David A. Czaplewski,Maxim Zalalutdinov,Harold G. Craighead,Pavel Neuzil,C. Campagnolo,Carl A. Batt +6 more
TLDR
A resonant frequency-based mass sensor, comprised of low-stress silicon nitride cantilever beams for the detection of Escherichia coli (E. coli)-cell-antibody binding events with detection sensitivity down to a single cell, is presented.Abstract:
The ability to detect small amounts of materials, especially pathogenic bacteria, is important for medical diagnostics and for monitoring the food supply. Engineered micro- and nanomechanical systems can serve as multifunctional, highly sensitive, immunospecific biological detectors. We present a resonant frequency-based mass sensor, comprised of low-stress silicon nitride cantilever beams for the detection of Escherichia coli (E. coli)-cell-antibody binding events with detection sensitivity down to a single cell. The binding events involved the interaction between anti-E. coli O157:H7 antibodies immobilized on a cantilever beam and the O157 antigen present on the surface of pathogenic E. coli O157:H7. Additional mass loading from the specific binding of the E. coli cells was detected by measuring a resonant frequency shift of the micromechanical oscillator. In air, where considerable damping occurs, our device mass sensitivities for a 15 μm and 25 μm long beam were 1.1 Hz/fg and 7.1 Hz/fg, respectively. ...read more
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Nanostructures in Biodiagnostics
Nathaniel L. Rosi,Chad A. Mirkin +1 more
TL;DR: Nathaniel L. Rosi focuses on the rational assembly of DNA-modified nanostructures into larger-scale materials and their roles in biodiagnostic screening for nucleic acids.
Journal ArticleDOI
Cantilever transducers as a platform for chemical and biological sensors
TL;DR: In this article, the authors discuss sensors with transducers in a form of cantilevers, which are especially attractive as transducers for chemical and biological sensors, and provide a brief analysis of historical predecessors of the modern cantilever sensors.
Journal ArticleDOI
Micro- and nanomechanical sensors for environmental, chemical, and biological detection
TL;DR: The technologies and recent developments in the field of micro- and nanoelectromechanical sensors with particular emphasis on their application as biological sensors and recent work towards integrating these sensors in microfluidic systems are reviewed.
Journal ArticleDOI
Attogram detection using nanoelectromechanical oscillators
Bojan Ilic,Harold G. Craighead,Slava Krylov,Wageesha Senaratne,Christopher K. Ober,Pavel Neuzil +5 more
TL;DR: In this article, the rotational inertia correction to the frequency is on the order of γ(l 0/l)2, where γ is the attached mass normalized to the mass of the beam.
Journal ArticleDOI
Single virus particle mass detection using microresonators with nanoscale thickness
TL;DR: In this article, the authors used arrays of silicon cantilever beams as microresonator sensors with nanoscale thickness to detect the mass of individual virus particles and demonstrated the detection of a single vaccinia virus particle with an average mass of 9.5 fg.
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Journal Article
Silicon as a mechanical material
TL;DR: In this article, the advantages of employing silicon as a mechanical material, the relevant mechanical characteristics of silicon, and the processing techniques which are specific to micromechanical structures are discussed.